c-Raf-1 (AA 162-378) antibody

Antigen: c-Raf-1

Binding Site: AA 162-378

Clonality: Monoclonal (53)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN967783

Additional Information

Immunogen: Human c-Raf-1

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 53

Description: Raf (c-Raf-1) is a cytoplasmic serine/threonine protein kinase and is a member of a family of proteins which are highly conserved from Drosophila to mammals. Raf has a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF. Raf can directly interact with Ras-GTP and subsequently become activated. Thus Raf plays a prominent role in the Ras signaling pathway by transferring a signal from Ras which has been activated by growth factor receptor-stimulated tyrosine kinase activity. This leads to the stimulation and activation of a number of other cytoplasmic serine/threonine kinases. Raf regulates the MAP kinase pathway by phosphorylating and activating MEK, which then phosphorylates and activates MAP kinase (ERK). ERK then phosphorylates and activates Rsk. Raf activity can also be regulated independently of Ras. Cyclic AMP (cAMP) activation of protein kinase A (PKA) can inhibit growth factor stimulation of Raf.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 74 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of c-Raf-1 on A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of c-Raf-1 antibody.

Immunofluorescent staining of A431 cells.

anti-c-Raf-1 (AA 162-378) antibody (Image 3)

Publications

Product: Rapp: "Role of Raf-1 serine/threonine protein kinase in growth factor signal transduction." in: Oncogene, Vol. 6, Issue 4, pp. 495-500, 1991 (PubMed).

Bondzi, Grant, Krystal: "A novel assay for the measurement of Raf-1 kinase activity." in: Oncogene, Vol. 19, Issue 43, pp. 5030-3, 2000 (PubMed).

Cheng, Wung, Chao et al.: "Sequential activation of protein kinase C (PKC)-alpha and PKC-epsilon contributes to sustained Raf/ERK1/2 activation in endothelial cells under mechanical strain." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 31368-75, 2001 (PubMed).

Rizzo, Kraft, Watkins et al.: "Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 34928-33, 2001 (PubMed).

Coles, Shaw: "PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway." in: Oncogene, Vol. 21, Issue 14, pp. 2236-44, 2002 (PubMed).