NOS1 antibody (AA 1095-1289)

Catalog No. ABIN967894

This Mouse Monoclonal antibody specifically detects NOS1 in WB, IHC, IP and IF. It exhibits reactivity toward Human, Rat, Mouse and Dog and has been mentioned in 5+ publications.

Quick Overview for NOS1 antibody (AA 1095-1289) (ABIN967894)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 16-nNOS-NOS Type I

Product Details anti-NOS1 Antibody

Binding Specificity: AA 1095-1289

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human nNOS aa. 1095-1289

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-NOS1 antibody (ABIN967894)

Noorian, Taylor, Annerino, Greene: "Neurochemical phenotypes of myenteric neurons in the rhesus monkey." in: The Journal of comparative neurology, Vol. 519, Issue 17, pp. 3387-401, (2012) (PubMed).

Schuh, Uldrijan, Telkamp, Rothlein, Neyses: "The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I." in: The Journal of cell biology, Vol. 155, Issue 2, pp. 201-5, (2001) (PubMed).

Sasaki, Gonzalez-Zulueta, Huang, Herring, Ahn, Ginty, Dawson, Dawson: "Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8617-22, (2000) (PubMed).

Yu, Shao, Qian, George, Rockey: "Gene transfer of the neuronal NO synthase isoform to cirrhotic rat liver ameliorates portal hypertension." in: The Journal of clinical investigation, Vol. 105, Issue 6, pp. 741-8, (2000) (PubMed).

Nathan: "Nitric oxide as a secretory product of mammalian cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 6, Issue 12, pp. 3051-64, (1992) (PubMed).

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: nNOS/NOS Type I

Background: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization. This antibody is routinely tested by western blot analysis.
Synonyms: NOS Type I, Neuronal Nitric Oxide Synthase

Molecular Weight: 155 kDa

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction