GRIP1 antibody (AA 877-1067)

Catalog No. ABIN968471

Product Details anti-GRIP1 Antibody

Target: GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))

Binding Specificity: AA 877-1067

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat GRIP aa. 877-1067

Clone: 32-GRIP

Isotype: IgG1

Application Details

Application Notes: Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-GRIP1 antibody (ABIN968471)

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Setou, Seog, Tanaka, Kanai, Takei, Kawagishi, Hirokawa: "Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites." in: Nature, Vol. 417, Issue 6884, pp. 83-7, (2002) (PubMed).

Bowery, Brown: "The cloning of GABA(B) receptors." in: Nature, Vol. 386, Issue 6622, pp. 223-4, (1997) (PubMed).

Dong, OBrien, Fung, Lanahan, Worley, Huganir: "GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors." in: Nature, Vol. 386, Issue 6622, pp. 279-84, (1997) (PubMed).

Target Details for GRIP1

Target: GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))

Alternative Name: GRIP (GRIP1 Products)

Synonyms: GRIP antibody, 4931400F03Rik antibody, eb antibody, grip antibody, xgrip1 antibody, GRIP1 antibody, si:dkey-244b23.1 antibody, si:dkey-6a5.1 antibody, glutamate receptor interacting protein 1 antibody, glutamate receptor interacting protein 1 S homeolog antibody, glutamate receptor-interacting protein 1 antibody, GRIP1 antibody, Grip1 antibody, grip1.S antibody, LOC525592 antibody, grip1 antibody

Background: Rapid neuronal excitation within the CNS is mediated by the interactions of receptors with their respective neurotransmitters, such as glutamate. Glutamate has a diverse array of receptors that are categorized into two distinct groups: ionotropic and metabotropic. Ionotropic receptors are ligand-gated channels and can be subdivided into two classes: NMDA and AMPA receptors. AMPA receptors are composed of four homologous subunits (GluR1-4) that differentially combine to form a variety of receptor subtypes. Interactions of the subunits with cytoplasmic proteins mediate the transmission of extracellular signals. GRIP (Glutamate Receptor Interacting Protein) specifically interacts with the C-terminus of the GluR2 subunit. GRIP lacks a catalytic domain, but contains seven PDZ domains which are motifs that mediate protein-protein interactions. Since only the fourth and fifth PDZ domains are utilized in interaction with GluR2, it is thought that the remaining PDZ domains interact with other unidentified proteins. Therefore, GRIP functions as an adaptor that links AMPA receptors to cytoskeletal and/or signaling molecules and, in turn, clusters them at the synapse.
Synonyms: Glutamate Receptor Interacting Protein

Molecular Weight: 130 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway