RAD9 Homolog A (S. Pombe) (RAD9A) (AA 264-370) antibody

Details for Product No. ABIN968473
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Antigen
Synonyms RAD9, Rad9, rad9a, MGC89062
Epitope
AA 264-370
(18), (17), (17), (16), (16), (13), (13), (12), (8), (6), (5), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(152), (61), (59), (24), (17), (14), (12), (12), (5), (4), (1)
Host
Mouse
(197), (11), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (10), (9), (9), (9), (9), (9), (9), (9), (9), (9), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(119), (90), (82), (46), (34), (14), (6), (5), (4), (3), (1)
Pubmed 5 references available
Catalog no. ABIN968473
Quantity 50 µg
Price
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Immunogen Human hRAD9
Clone 56
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name hRad9
Background Cell cycle checkpoints are regulatory mechanisms that prevent cell cycle progression in the presence of DNA damage or incompletely replicated DNA. Many of the genes required for cell-cycle arrest are also involved in DNA repair, apoptosis, and induction of transcription. In yeast and humans, hRAD9 plays a role in cell cycle arrest during the G2 phase before entry into mitosis. Phosphorylated hRAD9 is found in the nucleus after DNA damage, and forms DNA damage-responsive complexes with other putative checkpoint control proteins, such as hRAD1 and hHUS1. Expression of hRAD9 in S. pombe rad9::ura4+ cells restores resistance to the DNA synthesis inhibitor hydroxyurea and gamma rays. In addition, hRAD9 binds the anti-apoptotic proteins, Bcl-2 and Bcl-xL , and antisense hRAD9 RNA suppresses DNA-damage induced cell death. Thus, hRAD9 may be an important component of protein complexes that regulate cell cycle progression, as well as apoptosis, in response to DNA damage.
Molecular Weight 60 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 264-370) antibody Western blot analysis of hRAD9 on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-hRAD9 antibody.
anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 264-370) antibody (2) anti-RAD9 Homolog A (S. Pombe) (RAD9A) (AA 264-370) antibody (Image 2)
Product cited in: Lieberman, Hopkins, Nass et al.: "A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 13890-5, 1997 (PubMed).

Volkmer, Karnitz: "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 567-70, 1999 (PubMed).

St Onge, Udell, Casselman et al.: "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1." in: Molecular biology of the cell, Vol. 10, Issue 6, pp. 1985-95, 1999 (PubMed).

Komatsu, Miyashita, Hang et al.: "Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes apoptosis." in: Nature cell biology, Vol. 2, Issue 1, pp. 1-6, 2000 (PubMed).

Yoshida, Komatsu, Wang et al.: "c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3292-300, 2002 (PubMed).

Hosts (197), (11), (5)
Reactivities (152), (61), (59), (24), (17), (14), (12), (12), (5), (4), (1)
Applications (119), (90), (82), (46), (34), (14), (6), (5), (4), (3), (1)
Conjugates (10), (10), (9), (9), (9), (9), (9), (9), (9), (9), (9), (1), (1), (1)
Epitopes (18), (17), (17), (16), (16), (13), (13), (12), (8), (6), (5), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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