TNF Receptor-Associated Protein 1 (TRAP1) (AA 253-464) antibody
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|3 references available|
|Quantity||50 µg (250 µg/ml) (Variants)|
|Price||Product not available in this region.|
|Cross-Reactivity||Dog (Canine), Rat (Rattus), Mouse (Murine)|
The hsp90 family of molecular chaperones is involved in a number of signal transduction pathways, including cell division, protein folding and transport. A new member of this family was recently identified as TRAP1 (Tumor necrosis factor receptor-associated protein) or hsp75. TRAP1 is a mitochondrial protein that is an ATPase and ATP-binding protein, rendering it similar to hsp90. There is a high degree of homology between TRAP1 and hsp90 in 6 domains, however one major difference is that a highly charged domain present in hsp90 is absent in TRAP1. TRAP1 has been shown to interact with the intracellular domain of the type 1 receptor for tumor necrosis factor (TNFR-1IC) and to the retinoblastoma protein (Rb). Signaling through TNFR-1 induces antiviral responses, fibroblast proliferation, cytotoxicity, etc. TRAP1 binds TNFR-1IC outside of the death domain leading to disruption in the signaling by TNFR-1. Rb has been shown to play a pivotal role in cellular differentiation and progression through the cell cycle. TRAP1 associates with Rb via an LxCxE motif, which is common among proteins that interact with Rb through SV40 T-antigen binding domain. Thus, TRAP1 is a new member of the hsp90 family that interacts with Rb and TNFR-1IC.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||75 kDa|
Related Products: ABIN967389, ABIN968535
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
|Western blot analysis of Hsp75 on HeLa lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of Hsp75. Immunofluorescence staining of HeLa cells.|
Song, Dunbar, Zhang et al.: "Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor." in: The Journal of biological chemistry, Vol. 270, Issue 8, pp. 3574-81, 1995 (PubMed).
Chen, Chen, Dai et al.: "A new member of the hsp90 family of molecular chaperones interacts with the retinoblastoma protein during mitosis and after heat shock." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 4691-9, 1996 (PubMed).
Felts, Owen, Nguyen et al.: "The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3305-12, 2000 (PubMed).
|Hosts||Rabbit (33), Mouse (6)|
|Reactivities||Human (38), Rat (Rattus) (23), Mouse (Murine) (22), Chicken (19), Horse (Equine) (18), Dog (Canine) (2), Monkey (2)|
|Applications||Western Blotting (WB) (23), Immunofluorescence (IF) (19), ELISA (9), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (3), C-Term (1)|