GATA4 antibody

Catalog No. ABIN969169

This Mouse Monoclonal antibody specifically detects GATA4 in WB and ELISA. It exhibits reactivity toward Human and Rat and has been mentioned in 2+ publications.

Quick Overview for GATA4 antibody (ABIN969169)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 6H10

Product Details anti-GATA4 Antibody

Purpose: GATA4 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human GATA4 expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GATA4 antibody (ABIN969169)

Rajagopal, Ma, Obler, Shen, Manichaikul, Tomita-Mitchell, Boardman, Briggs, Garg, Srivastava, Goldmuntz, Broman, Benson, Smoot, Pu: "Spectrum of heart disease associated with murine and human GATA4 mutation." in: Journal of molecular and cellular cardiology, Vol. 43, Issue 6, pp. 677-85, (2007) (PubMed).

Belaguli, Sepulveda, Nigam, Charron, Nemer, Schwartz: "Cardiac tissue enriched factors serum response factor and GATA-4 are mutual coregulators." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7550-8, (2000) (PubMed).

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4

Background: GATA binding protein 4, also known as GATA4, it is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.

Molecular Weight: 46 kDa

Gene ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis