IHOG antibody

Catalog No. ABIN969211

This Mouse Monoclonal antibody specifically detects IHOG in ELISA. It exhibits reactivity toward Drosophila melanogasterand has been mentioned in 1 publication.

Quick Overview for IHOG antibody (ABIN969211)

Target: IHOG (Interference Hedgehog (IHOG))

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This IHOG antibody is un-conjugated

Application: ELISA

Clone: 3G8

Product Details anti-IHOG Antibody

Purpose: IHOG Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human IHOG expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-IHOG antibody (ABIN969211)

McLellan, Yao, Zheng, Geisbrecht, Ghirlando, Beachy, Leahy: "Structure of a heparin-dependent complex of Hedgehog and Ihog." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 46, pp. 17208-13, (2006) (PubMed).

Target Details for IHOG

Target: IHOG (Interference Hedgehog (IHOG))

Alternative Name: IHOG

Background: The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

Molecular Weight: 98 kDa

Gene ID: 33972

UniProt: Q9VM64