KARS antibody (AA 90-174)

Catalog No. ABIN969226

This Mouse Monoclonal antibody specifically detects KARS in IHC and ELISA. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for KARS antibody (AA 90-174) (ABIN969226)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KARS antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: 8G12C1

Product Details anti-KARS Antibody

Binding Specificity: AA 90-174

Purpose: KARS Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-KARS antibody (ABIN969226)

Sampath, Mazumder, Seshadri, Gerber, Chavatte, Kinter, Ting, Dignam, Kim, Driscoll, Fox: "Noncanonical function of glutamyl-prolyl-tRNA synthetase: gene-specific silencing of translation." in: Cell, Vol. 119, Issue 2, pp. 195-208, (2004) (PubMed).

Sang Lee, Gyu Park, Park, Seol, Lee, Kim: "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex." in: Biochemical and biophysical research communications, Vol. 291, Issue 1, pp. 158-64, (2002) (PubMed).

Cen, Khorchid, Javanbakht, Gabor, Stello, Shiba, Musier-Forsyth, Kleiman: "Incorporation of lysyl-tRNA synthetase into human immunodeficiency virus type 1." in: Journal of virology, Vol. 75, Issue 11, pp. 5043-8, (2001) (PubMed).

Tolkunova, Park, Xia, King, Davidson: "The human lysyl-tRNA synthetase gene encodes both the cytoplasmic and mitochondrial enzymes by means of an unusual alternative splicing of the primary transcript." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35063-9, (2000) (PubMed).

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: KARS

Background: KARS: lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Gene ID: 3735

UniProt: Q15046