Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody

Details for Product No. ABIN969517
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Antigen
Synonyms
CG10837, Dm-eIF4B, Dmel\\CG10837, EIF-4B, Eif4B, eIF-4B-L, eIF-4B-S, eIF-4b, eIF4B, eIF4B-L, eIF4B-S, eIf4b, eif4b, fb15c09, wu:fb15c09, zgc:101532, MGC89384, EIF4B, DKFZp469D171, PRO1843, 2310046H11R ... show more
CG10837, Dm-eIF4B, Dmel\\CG10837, EIF-4B, Eif4B, eIF-4B-L, eIF-4B-S, eIF-4b, eIF4B, eIF4B-L, eIF4B-S, eIf4b, eif4b, fb15c09, wu:fb15c09, zgc:101532, MGC89384, EIF4B, DKFZp469D171, PRO1843, 2310046H11Rik, AL024095, C85189, Eif4a2 show less
Reactivity
Human, Mouse (Murine)
(132), (59), (31), (25), (24), (12), (12), (1), (1), (1)
Host
Mouse
(118), (17), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (7), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(97), (52), (51), (30), (29), (17), (9), (4), (3), (1)
Pubmed 2 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN969517
396.00 $
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Immunogen Purified recombinant fragment of human EIF4B expressed in E. coli.
Clone 1F5
Isotype IgG2b
Purification Purified
Alternative Name EIF4B
Background Synonyms:
EIF-4B, PRO1843
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F
Molecular Weight 80 kDa
Gene ID 1975
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (2) Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red) .
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (3) Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (4) Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (5) Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Product cited in: Shahbazian, Roux, Mieulet et al.: "The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity." in: The EMBO journal, Vol. 25, Issue 12, pp. 2781-91, 2006 (PubMed).

Mokas, Mills, Garreau et al.: "Uncoupling stress granule assembly and translation initiation inhibition." in: Molecular biology of the cell, Vol. 20, Issue 11, pp. 2673-83, 2009 (PubMed).

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