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Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody

Details for Product No. ABIN969517, Supplier: Log in to see
Antigen
  • CG10837
  • Dm-eIF4B
  • Dmel\\CG10837
  • EIF-4B
  • Eif4B
  • eIF-4B-L
  • eIF-4B-S
  • eIF-4b
  • eIF4B
  • eIF4B-L
  • eIF4B-S
  • eIf4b
  • eif4b
  • fb15c09
  • wu:fb15c09
  • zgc:101532
  • MGC89384
  • EIF4B
  • DKFZp469D171
  • PRO1843
  • 2310046H11Rik
  • AL024095
  • C85189
  • Eif4a2
Reactivity
Human, Mouse (Murine)
158
41
14
12
2
2
2
2
1
1
Host
Mouse
148
23
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
8
8
8
7
7
7
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
136
90
75
25
24
12
6
5
3
3
Supplier
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Immunogen Purified recombinant fragment of human EIF4B expressed in E. coli.
Clone 1F5
Isotype IgG2b
Purification Purified
Alternative Name EIF4B (EIF4B Antibody Abstract)
Background The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F
Molecular Weight 80 kDa
Gene ID 1975
HGNC 1975
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (ABIN969517) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (ABIN969517) Figure 2: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (ABIN969517) Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green) ...
Western Blotting (WB) image for anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (ABIN969517) Figure 4: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), Hep...
Western Blotting (WB) image for anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (ABIN969517) Figure 5: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) rec...
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (ABIN969517) anti-Eukaryotic Translation Initiation Factor 4B (EIF4B) antibody (Image 6)
Product cited in: Mokas, Mills, Garreau et al.: "Uncoupling stress granule assembly and translation initiation inhibition." in: Molecular biology of the cell, Vol. 20, Issue 11, pp. 2673-83, 2009 (PubMed).

Shahbazian, Roux, Mieulet et al.: "The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity." in: The EMBO journal, Vol. 25, Issue 12, pp. 2781-91, 2006 (PubMed).