Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 1-333) antibody

Antigen: Class II, Major Histocompatibility Complex, Transactivator (CIITA)

Synonyms: C2TA, NLRA, MHC2TA, CIITAIV, C2ta, Mhc2ta, CIITA

Epitope: AA 1-333

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN97339

Additional Information

Alternative name: CIITA (AA 1-333)

Immunogen: The immunogen used for this study was a bacterially produced recombinant FLAG-CIITA corresponding to amino acids 1 through 333 of the human protein.

Format: Liquid (sterile filtered)

Description: The transactivator CIITA regulates basal and interferon-induced expression of Major Histocompatibility Complex class II genes. CIITA restores expression of all MHC class II gene expression in mutant cells and corrects regulatory defects of MHC class II genes. Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression. Antigen can be obtained from Raji cell lysates. Typically levels of CIITA expression are too low to detect endogenous levels of protein expression. Transiently transfected cells are usually employed to study this transcription factor.

Application Details

Application Notes: Recommended Dilutions: For immunoblotting a 1:500 dilution is recommended. Researchers should determine optimal titers for other applications. Suitable for immunoblotting, ELISA and immunoprecipitation.

Concentration: 90.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.

Research Area: Transcription Factors

Restrictions: For Research Use only

Images

Immunoblot of anti-CIITA antibodies shows specificity. Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates of Cos-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA. For transfection, Fugene 6 (Roche) was used according to the manufacturer's instructions for a 6-well plate format. Cells were lysed 24 h posttransfection in 200 uL of 1x SDS-sample buffer, heated at 96

Immunoblot of anti-CIITA antibodies shows sensitivity. Anti-CIITA (1-333) antibody, generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates from Spodoptera frugiperda 21 (SF21) cells (2 x 105 cells in 200 uL of 1x SDSsample buffer) and an SF21 lysate spiked with recombinant FLAG-CIITA to 0.2 ng/uL. Cells from both preparations were lysed by heating at 96

Publications

Publications: Koues, Dudley, Mehta et al.: "The 19S proteasome positively regulates histone methylation at cytokine inducible genes." in: Biochimica et biophysica acta, Vol. 1789, Issue 11-12, pp. 691-701, 2009 (PubMed).