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14-3-3 zeta antibody (Ser58)

This Rabbit Polyclonal antibody specifically detects 14-3-3 zeta in WB and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN197404
$588.00
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Quick Overview for 14-3-3 zeta antibody (Ser58) (ABIN197404)

Target

See all 14-3-3 zeta (YWHAZ) Antibodies
14-3-3 zeta (YWHAZ)

Reactivity

  • 142
  • 84
  • 68
  • 11
  • 10
  • 8
  • 8
  • 8
  • 8
  • 5
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Human, Mouse

Host

  • 126
  • 16
Rabbit

Clonality

  • 127
  • 15
Polyclonal

Conjugate

  • 80
  • 10
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  • 6
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This 14-3-3 zeta antibody is un-conjugated

Application

  • 128
  • 62
  • 60
  • 35
  • 34
  • 19
  • 13
  • 13
  • 11
  • 10
  • 3
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 30
    • 20
    • 9
    • 9
    • 7
    • 7
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
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    • 1
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    • 1
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    • 1
    Ser58

    Specificity

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous levels of total 14-3-3 zeta protein.

    Purification

    Immunoaffinity Chromatography.

    Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-Sp-W-R).
  • Application Notes

    Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Store the antibody (in aliquots) at -20 °C.
  • Target

    14-3-3 zeta (YWHAZ)

    Alternative Name

    14-3-3 Protein zeta/delta

    Background

    Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ

    Gene ID

    7534

    NCBI Accession

    NP_001129171

    UniProt

    P63104

    Pathways

    Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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