NR0B1 antibody (Internal Region)
Quick Overview for NR0B1 antibody (Internal Region) (ABIN190789)
Target
See all NR0B1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- Internal Region
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Purpose
- DAX1 / NR0B1
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Sequence
- CGEDHPQQGS TLY
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Cross-Reactivity
- Human
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence CGEDHPQQGSTLY, from the internal region of the protein sequence according to NP_000466.2.
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Isotype
- IgG
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anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (Internal Region) antibody
NR0B1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 1-227) antibodyNR0B1 Reactivity: Mouse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 1-470) antibodyNR0B1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 361-470) antibodyNR0B1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1F10 unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 1-243) antibodyNR0B1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 1-260) antibodyNR0B1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 304-333) antibodyNR0B1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB33887 unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) antibodyNR0B1 Reactivity: Human, Mouse WB, IF Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (AA 361-470) antibodyNR0B1 Reactivity: Human ELISA, IF Host: Mouse Monoclonal 2F12 unconjugated
anti-Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) (Ligand Binding Domain) antibodyNR0B1 Reactivity: Human IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
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Application Notes
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Western Blot: Approx 55 kDa band observed in Human Adrenal Gland lysates (calculated MW of 51.7 kDa according to NP_000466.2). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
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Comment
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))
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Alternative Name
- NR0B1
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Background
- NR0B1, DAX1, nuclear receptor subfamily 0, group B, member 1, AHC, AHCH, AHX, DAX-1, DSS, GTD, HHG, NROB1 , dosage-sensitive sex reversal, gonadotropin deficiency, nuclear hormone receptor
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Gene ID
- 190
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NCBI Accession
- NP_000466
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Pathways
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
Target
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