RYBP antibody
Quick Overview for RYBP antibody (ABIN155069)
Target
See all RYBP AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Specificity
- This antibody detects DEDAF. A 32 kDa band should be detected.
-
Purification
- Affinity purified
-
Immunogen
- Synthetic peptide surrounding amino acid 218 of human DEDAF.
-
-
anti-RING1 and YY1 Binding Protein (RYBP) (AA 1-228) antibody
RYBP Reactivity: Human WB, ELISA, IP Host: Mouse Monoclonal 1F4 unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (AA 1-228) antibodyRYBP Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (AA 1-228) antibodyRYBP Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (AA 215-228) antibodyRYBP Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Monkey, Pig, Chicken, Hamster WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (AA 71-120) antibodyRYBP Reactivity: Human, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Monkey, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (N-Term) antibodyRYBP Reactivity: Human, Mouse, Zebrafish (Danio rerio), Xenopus laevis WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) antibodyRYBP Reactivity: Human WB, IF Host: Mouse Monoclonal 4E10 unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) antibodyRYBP Reactivity: Human WB, FACS Host: Mouse Monoclonal 1B6 unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (Middle Region) antibodyRYBP Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated
anti-RING1 and YY1 Binding Protein (RYBP) (C-Term) antibodyRYBP Reactivity: Human, Mouse WB Host: Rabbit Polyclonal unconjugated
-
-
Application Notes
-
Western Blot: 0.5-4 μg/mL. A549 or HepG2 cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Concentration
- 0.5 mg/mL
-
Buffer
- PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal
-
Preservative
- Thimerosal (Merthiolate)
-
Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- Avoid repeated freezing and thawing.
-
Storage
- -20 °C/-80 °C
-
Storage Comment
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
-
-
- RYBP (RING1 and YY1 Binding Protein (RYBP))
-
Alternative Name
- RYBP
-
Background
- Cell death signal are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA biding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.Synonyms: AAP-1, APAP-1, Apoptin-associating protein 1, DED-associated factor, DEDAF, Death effector domain-associated factor, RING1 and YY1-binding protein, YEAF1, YY1 and E4TF1-associated factor 1
-
Gene ID
- 23429
-
NCBI Accession
- NP_036366
-
UniProt
- Q8N488
Target
-
