Mouse anti-Human Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (Joining Chain) Antibody
IGJ
WB, EIA, IP, IHC (fro)
Monoclonal
MC19-9
IgG1
unconjugated
(1 reference)
Catalog No. ABIN118394
$852.00
Plus shipping costs $50.00
0.2 mg
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Quick Overview for Mouse anti-Human Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (Joining Chain) Antibody (ABIN118394)
Target
IGJ
(Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))
Clonality
Monoclonal
Application
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
Clone
MC19-9
Isotype
IgG1
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Binding Specificity
- Joining Chain
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Reactivity
- Human
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Host
- Mouse
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Specificity
- The antibody reacts with Human J chain as tested by ELISA using purified Human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques SM1013P detects J chain in IgM only in reducing conditions.
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Purification
- Affinity Chromatography on Protein A.
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Immunogen
- Human J Chain. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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Application Notes
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Immunohistochemistry on Frozen Sections (1/200). ELISA (20 μg/mL). Immunoprecipitation (20 μg/mL). Western blotting (only under Reducing Conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- TRIS buffered Saline containing 0.09 % Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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: "Isotype-switched immunoglobulin genes with a high load of somatic hypermutation and lack of ongoing mutational activity are prevalent in mediastinal B-cell lymphoma." in: Blood, Vol. 98, Issue 9, pp. 2762-70, (2001) (PubMed).
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- IGJ (Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))
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Alternative Name
- Immunoglobulin J Chain
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Background
- Immunoglobulin J chain (IGJ) serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory components.Synonyms: IGCJ, IGJ
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Gene ID
- 3512
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NCBI Accession
- NP_653247
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UniProt
- P01591
Target
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