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KPNA2 antibody (C-Term)

The Goat Polyclonal anti-KPNA2 antibody (ABIN185157) specifically detects KPNA2 in WB, IHC, ELISA, IF and FACS. The antibody is reactive with Human and Rat samples.
Catalog No. ABIN185157
$488.25
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days

Quick Overview for KPNA2 antibody (C-Term) (ABIN185157)

Target

See all KPNA2 Antibodies
KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Reactivity

  • 67
  • 37
  • 32
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Rat

Host

  • 48
  • 14
  • 2
  • 2
  • 1
Goat

Clonality

  • 34
  • 33
Polyclonal

Conjugate

  • 39
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
This KPNA2 antibody is un-conjugated

Application

  • 47
  • 16
  • 15
  • 13
  • 12
  • 12
  • 5
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade

Verified
  • Binding Specificity

    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purpose

    KPNA2 / IPOA1

    Sequence

    QVQDGAPGTF NF

    Cross-Reactivity

    Dog, Human, Mouse, Rat

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-QVQDGAPGTFNF, from the C Terminus of the protein sequence according to NP_002257.1.

    Isotype

    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 55 kDa band observed in lysates of cell lines CaCo-2, Jurkat, and KNRK, and approx. 58 kDa in lysates of cell lines MCF7 and A549 (calculated MW of 57.9 kDa according to Human NP_002257.1 and Rat NP_001257731.1). Recommended concentra

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in U2OS and A549 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

    Alternative Name

    KPNA2

    Background

    KPNA2, IPOA1 QIP2, RCH1, SRP1alpha, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), RAG cohort 1, importin alpha 1, importin alpha 2, importin-alpha-P1, karyopherin alpha 2, pendulin

    Gene ID

    3838, 16647, 85245

    NCBI Accession

    NP_002257

    Pathways

    M Phase, Protein targeting to Nucleus
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