Neutrophils antibody (PE)
Quick Overview for Neutrophils antibody (PE) (ABIN114284)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Characteristics
- Absorption / Emission: 488 nm / 575 nm
-
Purification
- Protein A affinity purified Ig
-
Immunogen
- Cultured bone marrow cells
-
Isotype
- IgG2a
-
-
-
-
Application Notes
-
Flow cytometry (see protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protocol
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation using an NH4Cl lysing buffer.
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 0.1 mg/mL
-
Buffer
- PBS with 0.09 % NaN3 as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- Avoid repeated freezing and thawing.
-
Storage
- -20 °C
-
Storage Comment
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
-
Expiry Date
- 6 months
-
-
- Neutrophils
Target
-