Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA Kit

Antigen: Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1)

Synonyms: CLG, CLGN, Dm1-MMP, l(2)k04809, mmp1, DmelCG4859, CG4859, MMP1, Mmp1a, Mmp1

Reactivity: Human

Application: ELISA

Catalog no.: ABIN411327

Additional Information

Characteristics: For quantitative detection of human MMP-1 in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Alternative name: Matrix Metalloproteinase 1 (MMP1)

Description: Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases.1 MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema.2 Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking.3 The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 20pg/ml. Range: 156pg/ml-10,000pg/ml

Application Details

Principle: This human MMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-1 amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant human MMP-1standard: 10ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

Images

Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA Kit