Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA Kit

Antigen: Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE)

Synonyms: DCP, ACE1, DCP1, CD143, MVCD3, MGC26566, AW208573, MGC117827, Dcp1, StsRR92, ACE, LOC100009274, dcp, ace1, dcp1, xace, cd143

Reactivity: Human

Application: ELISA

Catalog no.: ABIN411373

Additional Information

Characteristics: For quantitative detection of human ACE in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Alternative name: Angiotensin-converting enzyme (ACE)

Description: Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073.1 Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm.2 The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 5 pg/ml. Range: 156pg/ml-10,000pg/ml

Application Details

Principle: This human ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human ACE specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ACE amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. EDTA and citrate are not recommended as the anticoagulant. Collect plasma using heparin as an anticoagulant. A complete protocol is available upon request.

Components: Lyophilized recombinant human ACE standard: 10ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

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Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) ELISA Kit