Cathepsin B (CTSB) ELISA Kit

Antigen: Cathepsin B (CTSB)

Synonyms: APPS, CPSB, apps, cpsb, MGC53360, LOC692390, Catb-2744, ACYPI000009, catb-1674a, Catb-84, ACYPI000014, Catb-3098, ACYPI000017, Catb-16d2, catb-16D, ACYPI000018, Catb-1874, ACYPI000019, Catb-3483, ACYPI000020, Catb-10270, ACYPI000021, CTSB, DKFZp459D187

Reactivity: Human

Application: ELISA

Catalog no.: ABIN411391

Additional Information

Characteristics: For quantitative detection of human Cathepsin B in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Description: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 5pg/ml. Range: 156pg/ml-10,000pg/ml

Synonyms: APPS, CPSB, apps, cpsb, MGC53360, LOC692390, Catb-2744, ACYPI000009, catb-1674a, Catb-84, ACYPI000014, Catb-3098, ACYPI000017, Catb-16d2, catb-16D, ACYPI000018, Catb-1874, ACYPI000019, Catb-3483, ACYPI000020, Catb-10270, ACYPI000021, CTSB, DKFZp459D187

Application Details

Principle: This human Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Cathepsin B specific-specific monoclonal antibodies(clone No.155714) were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin B amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant human Cathepsin B standard: 10ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

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Cathepsin B (CTSB) ELISA Kit