Albumin (ALB) ELISA Kit

Antigen: Albumin (ALB)

Synonyms: ALB, MGC179277, alb, pro0883, pro0903, pro1341, MGC88928, CSA, DKFZp459F2310, PRO0883, PRO0903, PRO1341, DKFZp779N1935, Alb1, Alb-1, Albza, LOC100136344

Reactivity: Mouse (Murine)

Catalog no.: ABIN454013

Additional Information

Alternative name: Albumin

Description: When blood is filtered in glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin appear in urine. Albumin therefore serves as a marker in monitoring kidney damages. In examining nephrotoxic substances and therapeutic strategies regarding kidney damages, animal models are frequently used. This ELISA allows quantification of albumin in mouse urine, so that an estimation of kidney function is possible. For determination of albumin in rat urine, another ELISA is available (albumin(rat)-ELISA, catalog no. 50100).

Sensitivity: 7 standards between 2.5 ng/ml and 10240 ng/ml Sensitivity:0.1 µg/ml (sample dilution 1:40) Sample material:Urine Intraassay precision (CV):(sample dilution 1:100, n=12) at 3.1 µg/ml: 14.3%at 30.4 µg/ml: 6.1% at 223 µg/ml: 5.8%

Application Details

Principle: Determination of albumin is carried out as direct competitive ELISA. Mouse albumin has been pre-coated onto a microtiter plate. During incubation binding of an enzyme-linked anti-albumin(mouse)-antibody is inhibited by albumin from samples/ standards. After washing away any unbound antibody a substrate solution is added. Developing colour correlated with the amount of antibody conjugate. Absorption at 450 nm is reverse proportional to albumin concentration.

Protocol: Incubation of samples/standards with antibody conjugate 50 µl + 50 µl/well 90 min with shaking,120 min without shaking. Wash Substrate incubation 100 µl/well 10 min, room temperature.. Add stop solution 100 µl/well. Read at 450 nm

Restrictions: For Research Use only

Publications

Publications: Heikkilae, Juhila, Lassila et al.: "beta-Catenin mediates adriamycin-induced albuminuria and podocyte injury in adult mouse kidneys." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 25, Issue 8, pp. 2437-46, 2010 (PubMed).

Hyvoenen, Saurus, Wasik et al.: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, 2010 (PubMed).