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anti-b2 adrenergic Receptor Antibodies (IgG) ELISA Kit
| Antigen | Anti-B2 Adrenergic Receptor Antibodies |
| Catalog no. | ABIN454022 |
| Quantity | 96 tests |
| Price | 1,885.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 3 to 5 Business Days |
Additional Information
| Alternative name | anti-b2 adrenergic Receptor Antibodies (IgG) |
| Description | The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma. |
| Sensitivity | 5 standards between 2,5 U/ml and 40 U/mlcut off:18 U/ml Sample materials:Serum, Plasma Intraassay-Precision:7.34% Interassay-Precision:10.82% |
Application Details
| Principle | The anti-alpha2-adrenergic-receptor-antibody-EIA is an antibody screening test. The alpha2-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-alpha2-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-alpha2-adrenergic-receptor-antibodies. |
| Protocol | Incubation of samples/ standards/ controls 100 µl 120 min, 4°C. Wash Incubation of detection antibody 100 µl 1 hr, room temperature. Wash Substrate incubation 100 µl/well 20 min, room temperature. Add Stopp solution 100 µl/well. Read at 450 nm |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
