IgM ELISA Kit
Quick Overview for IgM ELISA Kit (ABIN454030)
Target
See all IgM ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Minimum Detection Limit
- 98 ng/mL
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Purpose
- The IgM(chicken)-ELISA is designed for the quantitative determination of chicken IgM in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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Characteristics
- The determination of chicken IgM is carried out as direct sandwich ELISA.
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IgM ELISA Kit
Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Chicken Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
IgM ELISA KitReactivity: Human Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Grouper (Epinephelinae) Colorimetric Competition ELISA 1.25-50 μg/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Sheep Colorimetric Competition ELISA 2.5 μg/mL - 40 μg/mL Plasma, Serum
IgM ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgM ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum
IgM ELISA KitReactivity: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Rat Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Plasma, Serum
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Plate
- Pre-coated
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Protocol
- An antibody specific for chicken IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.
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Assay Procedure
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
Assay Precision
- Intraassay precision (CV):(n=10)at 7.4 ng/ml: 3.1%at 43.8 ng/ml: 6.1%at 147.3 ng/ml: 11.3%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IgM
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Target Type
- Antibody
Target See all IgM ELISA Kits
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