Kallikrein-Related Peptidase 3 (KLK3) ELISA Kit

Antigen: Kallikrein-Related Peptidase 3 (KLK3)

Synonyms: APS, PSA, hK3, KLK2A1, KLK3, KLKB1

Reactivity: Human

Application: ELISA

Certificates: ISO 13485:2003

Catalog no.: ABIN577067

Additional Information

Alternative name: Prostate Specific Antigen (PSA)

Description: Hepatitis resulting from infection with viruses other than Hepatitis A Virus (HAV) and Hepatitis B (HBV) virus was previously referred to as non-A, non-B hepatitis. The first characterised non-A, non-B hepatitis agent was that responsible for parentally transmitted non-A, non-B hepatitis, or what is now called Hepatitis C Virus. This was followed by the cloning of a portion of the fecal-orally-transmitted agent, the Hepatitis E Virus (HEV). Hepatitis E Virus has been referred to as enterically transmitted non-A, non-B hepatitis. Epidemics of enterically transmitted Hepatitis E Virus have been recognised worldwide but occur principally in developing countries. They have been reported in Southeast Asia, central Asia, Africa, Mexico, and Central America. In these areas, contaminated water has been implicated as the principal vehicle of virus transmission. Although HEV and HAV are transmitted in a similar manner, there are major differences in the clinical, pathological, and epidemiological courses of these two viruses. In particular, the mortality rate for HEV infection is 1 to 2%, or approximately 1-fold greater than that seen for HAV. Infection with HEV is particularly fatal for pregnant women, for whom the mortality rate can be as high as 1 to 2%

Sensitivity: Estimated to be 1 ng/mL PSA antigen in human serum.

Application Details

Principle: This PSA enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for PSA. Standards or samples are then added to the microtiter plate wells and PSA, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of PSA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody, specific for PSA are added to each well to "sandwich" the PSA immobilized on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PSA and enzyme-conjugated antibody will exhibit a change in colour. The enzyme- substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is e m asured spectrophotometrically at a wavelength of 450nm 2nm. In order to measure the concentration of PSA in the sample, this Human PSA ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples allowing the operator to produce a standard curve of Optical Density (O.D.) versus PSA concentration (ng/mL). The concentration of PSA in the samples is then determined by comparing the O.D. of the samples to the standard curve. S7.5(04) PSA 2

Restrictions: For Research Use only