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Principle
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The DetectX® Corticosterone Immunoassay kit is designed to quantitatively measure Corticosterone present in extracted dried fecal samples, serum, plasma and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
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