ERG Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720464

This Recombinant ERG protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for ERG Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2720464)

Target: ERG (ERG, ETS transcription factor (ERG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This ERG protein is labelled with Myc-DYKDDDDK Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human ERG (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Biological Activity Comment: Varying amounts of human ERG expressed in HEK293 cells was incubated for one hour with wild-type or mutant biotinylated oligonucleotide (1 pmole/ul) in the presence of 25 ug/ml poly dI:dC. The reaction mixture was subsequently transferred to a microplate containing 2500 Luminex beads coupled with anti-ERG monoclonal antibody 2G8. The ERG-oligo complexes were captured onto the antibody-coated beads for two hours at room temperature with shaking. The beads were then washed, and the biotin was detected with streptavidin-phycoerythrin for 30 minutes. The beads were washed again and the fluorescent intensity was read in the Luminex instrument. The wild-type oligonucleotide carried ACCGGAAGT consensus binding sequence while the mutant oligonucleotide was identical except for a 2-base mutation in the consensus binding region, ACCCCAAGT

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

He, Schepmoes, Shi, Wu, Fillmore, Gao, Smith, Qian, Rodland, Liu, Camp, Rastogi, Tan, Yan, Mohamed, Huang, Banerjee, Kagan, Srivastava, McLeod, Srivastava, Petrovics, Dobi, Srinivasan: "Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods." in: Journal of translational medicine, Vol. 13, pp. 54, (2015) (PubMed).

Target Details

Target: ERG (ERG, ETS transcription factor (ERG))

Alternative Name: Erg

Background: Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 53.7 kDa

NCBI Accession: NP_891548