Immunoglobulin E (IgE) Protein

Name: Immunoglobulin E (IgE)

Source: Human

Origin (Gene): Human

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Catalog no.: ABIN363702

Additional Information

Gene ID: IGHE, chromosome 14q32.33

Source (Details): Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system)

Format: Affinity-purified, Low-endotoxin, azide-free (LEAF)

Isotype: IgE lambda

Clone: SUS11-NBS01

Description: Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.

Specificity: Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown

Application Details

Application Notes: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100 Quality assurance: Reactivity > 99%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Concentration: 1mg/ml

Purity: > 95%, tested by analytical Gel Permeation Chromatography

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Storage: Store undiluted at 4°C. For long term storage, aliquot and store at -20°C

Research Area: Allergy

Restrictions: For Research Use only

Images

product purity: gel permeation chromatography (Superose 12/HR)

Target binding kinetics (Biacore

binding of increasing amounts of IgE(SUS-11) to recombinant rat RBL2H3 mast cells expressing the human high affinity IgE receptor

Publications

Publications: Zuercher, Lang, Aebischer et al.: "IgE-producing hybridomas established after B-cell culture in the CD40 system." in: Immunology letters, Vol. 46, Issue 1-2, pp. 49-57, 1995 (PubMed).

Pachlopnik, Staempfli, Rudolf et al.: "Antigen interaction and heat inactivation expose new epitopes on human IgE." in: International archives of allergy and immunology, Vol. 117, Issue 4, pp. 231-8, 1999 (PubMed).