Albumin (ALB) (Cohn Fraction V) protein (FITC)

Name: Albumin (ALB)

Synonyms: ALB, MGC179277, alb, pro0883, pro0903, pro1341, MGC88928, CSA, DKFZp459F2310, PRO0883, PRO0903, PRO1341, DKFZp779N1935, Alb1, Alb-1, Albza, LOC100136344

Epitope: Cohn Fraction V

Source: Bovine

Origin (Gene): Cow (Bovine)

Conjugate: FITC

Application: Counterstain (CS)

Catalog no.: ABIN457597

Additional Information

Characteristics: Fluorescein isothiocyanate-conjugated bovine serum albumin, Cohn fraction V

Source (Details): Source: Pooled normal bovine serum

Description: Confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to bovine total serum proteins, to bovine IgA, IgM and albumin

Application Details

Application Notes: As an efficient counterstain for cell an tissue substrates with immunoconjugates with a label contrasting with the greenish-yellow fluorescence of BSA/FITC. The purpose of counterstaining is to enhance the optical contrast of the immuno-specific staining against a counterstained background.

Concentration: BSA concentration 10 mg/ml. Fluorochrome/BSA molar ratio (F/P) 1.2.

Purity: Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immuno-precipitating techniques, this product contains traces transferrin and some alpha-proteins.

Purification: according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immuno-precipitating techniques, this product contains traces transferrin and some alpha-proteins.

Buffer: Fluorochrome-coupled bovine serum albumin, fraction V, lyophilized from a solution in phosphate buffered saline (PBS pH 7.2). Preservative: No preservative added.

Storage: Lyophilized at +4° C reconstituted at or below -20° C reconstituted at +4° C at least 10 years 3-5 years 7 days. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Restrictions: For Research Use only