Protein G Protein

Catalog No. ABIN621060

This Recombinant Protein G protein is expressed in not_set.

Quick Overview for Protein G Protein (ABIN621060)

Target: Protein G

Protein Type: Recombinant

Origin: Streptococcus

Host: Please inquire

Application: Detection (D), Quantification (Q), Purification (Purif)

Purity: > 95 % as determined by SDS-PAGE and RP-HPLC.

Product Details

Sequence: MTYKLILNGK TLKGETTTEA VDAATAEKVF KQYANDNGVD GEWTYDDATK TFTVTEKPEV IDASELTPAV TTYKLVINGK TLKGETTTEA VDAATAEKVF KQYANDNGVD GEWTYDDATK TFTVTEKPEV IDASELTPAV TTYKLVINGK TLKGETTTKA VDAETAEKAF KQYANDNGVD GVWTYDDATK TFTVTE

Specificity: 1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a. 2. Does not bind to human IgM, IgD and IgA.

Characteristics: Recombinant Protein G

Application Details

Application Notes: Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water or PBS

Buffer: Lyophilized white Powder containing no additives.

Storage: -20 °C

Target Details

Target: Protein G

Background: Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains amino acids 190-384 having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 32 kDa.