Diazepam Binding Inhibitor Protein (DBI) (AA 1-87) (His tag)
Quick Overview for Diazepam Binding Inhibitor Protein (DBI) (AA 1-87) (His tag) (ABIN667004)
Target
See all Diazepam Binding Inhibitor (DBI) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-87
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Purification tag / Conjugate
- This Diazepam Binding Inhibitor protein is labelled with His tag.
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Sequence
- MSQAEFEKAA EEVRHLKTKP SDEEMLFIYG HYKQATVGDI NTERPGMLDF TGKAKWDAWN ELKGTSKEDA MKAYINKVEE LKKKYGI
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Characteristics
- ACBP, 1-87aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Diazepam Binding Inhibitor (DBI) (AA 1-104) protein (GST tag)
DBI Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Diazepam Binding Inhibitor (DBI) (AA 1-87) protein (His-IF2DI Tag)DBI Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Diazepam Binding Inhibitor (DBI) (AA 1-87) protein (His tag)DBI Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Diazepam Binding Inhibitor (DBI) (AA 1-87) protein (His tag)DBI Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Diazepam Binding Inhibitor (DBI) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DBI Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Diazepam Binding Inhibitor (DBI)
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Alternative Name
- ACBP/DBI
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Background
- ACBP, also known as ACBD1, is a diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. Expression of ACBP is significantly induced during adipocyte differentiation. ACBP is a target for proliferator-activated receptor (PPAR) gamma, and is directly activated by PPARgamma/RXRalpha and PPARalpha/RXRalpha, but not by PPARdelta/RXRalpha. Recombinant human ACBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 12.2 kDa (107aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001073331
Target
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