MAT2A Protein (AA 1-395) (His tag)
Quick Overview for MAT2A Protein (AA 1-395) (His tag) (ABIN667082)
Target
See all MAT2A ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-395
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Purification tag / Conjugate
- This MAT2A protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MNGQLNGFHE AFIEEGTFLF TSESVGEGHP DKICDQISDA VLDAHLQQDP DAKVACETVA KTGMILLAGE ITSRAAVDYQ KVVREAVKHI GYDDSSKGFD YKTCNVLVAL EQQSPDIAQG VHLDRNEEDI GAGDQGLMFG YATDETEECM PLTIVLAHKL NAKLAELRRN GTLPWLRPDS KTQVTVQYMQ DRGAVLPIRV HTIVISVQHD EEVCLDEMRD ALKEKVIKAV VPAKYLDEDT IYHLQPSGRF VIGGPQGDAG LTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW VAKSLVKGGL CRRVLVQVSY AIGVSHPLSI SIFHYGTSQK SERELLEIVK KNFDLRPGVI VRDLDLKKPI YQRTAAYGHF GRDSFPWEVP KKLKY
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Characteristics
- MAT2A, 1-395aa, Human, His tagged, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-395) protein (His tag)
custom-made MAT2A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-395) protein (His tag)custom-made MAT2A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-395) protein (GST tag)MAT2A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Methionine Adenosyltransferase II, alpha (MAT2A) protein (Myc-DYKDDDDK Tag)MAT2A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))
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Alternative Name
- MAT2A
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Background
- Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer. Recombinant MAT2A, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques
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Molecular Weight
- 45.8 kDa (415aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_005902
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Pathways
- Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process
Target
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