HIV-1 Tat Interactive Protein 2, 30kDa (HTATIP2) (AA 1-242) protein (His tag)

Catalog No. ABIN667152

This Recombinant HIV-1 Tat Interactive Protein 2, 30kDa protein is expressed in Escherichia coli (E. coli).

Quick Overview for HIV-1 Tat Interactive Protein 2, 30kDa (HTATIP2) (AA 1-242) protein (His tag) (ABIN667152)

Target: HIV-1 Tat Interactive Protein 2, 30kDa (HTATIP2)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-242

Purification tag / Conjugate: His tag

Sequence: MGSSHHHHHH SSGLVPRGSH MAETEALSKL REDFRMQNKS VFILGASGET GRVLLKEILE QGLFSKVTLI GRRKLTFDEE AYKNVNQEVV DFEKLDDYAS AFQGHDVGFC CLGTTRGKAG AEGFVRVDRD YVLKSAELAK AGGCKHFNLL SSKGADKSSN FLYLQVKGEV EAKVEELKFD RYSVFRPGVL LCDRQESRPG EWLVRKFFGS LPDSWARGHS VPVVTVVRAM LNNVVRPRDK QMELLENKAI HDLGKAHGSL KP

Characteristics: HTATIP2, 1-242aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: HIV-1 Tat Interactive Protein 2, 30kDa (HTATIP2)

Alternative Name: TIP30/HTATIP2

Background: HTATIP2, also known as TIP30, is a member of the short-chain dehydrogenases/reductases (SDR) family. It acts as a tumor suppressor participating in metabolic suppression, inhibition of angiogenesis and induces the expression of apoptosis related genes Bad and Siva. HTATIP2 interacts with the activation domain of HIV-1 TAT and enhances its transcription by phosphorylating RNA polymerase II (Pol II). Defects in This protein are associated with hepatocellular carcinomas and apoptotic resistant tumor cells, suggesting a potential use for HTATIP2 in antitumor therapy. Recombinant human HTATIP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Molecular Weight: 29.3 kDa (262aa) confirmed by MALDI-TOF