NANP Protein (AA 1-248) (His tag)
Quick Overview for NANP Protein (AA 1-248) (His tag) (ABIN667232)
Target
Protein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-248
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Purification tag / Conjugate
- This NANP protein is labelled with His tag.
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Sequence
- MGLS RVRAVFFDLD NTLIDTAGAS RRGMLEVIKL LQSKYHYKEE AEIICDKVQV KLSKECFHPY NTCITDLRTS HWEEAIQETK GGAANRKLAE ECYFLWKSTR LQHMTLAEDV KAMLTELRKE VRLLLLTNGD RQTQREKIEA CACQSYFDAV VVGGEQREEK PAPSIFYYCC NLLGVQPGDC VMVGDTLETD IQGGLNAGLK ATVWINKNGI VPLKSSPVPH YMVSSVLELP ALLQSIDCKV SMST
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Characteristics
- NANP, 1-248aa, Human, His tag, E.coli
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Purification
- > 90% by SDS-PAGE
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N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) protein (His tag)
custom-made NANP Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) protein (His tag)custom-made NANP Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) protein (GST tag)NANP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
N-Acetylneuraminic Acid Phosphatase (NANP) (AA 1-248) protein (His-IF2DI Tag)NANP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
N-Acetylneuraminic Acid Phosphatase (NANP) protein (Myc-DYKDDDDK Tag)NANP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 2 mM DTT, 100 mM NaCl
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- NANP (N-Acetylneuraminic Acid Phosphatase (NANP))
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Alternative Name
- HDHD4/NANP
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Background
- NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating N-acylneuraminate 9-phosphate to form N-acylneuraminate (N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate). Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium. Recombinant human NANP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 31.9 kDa (284aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_689880
Target
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