NT5C2 Protein (AA 1-561) (His tag)
Quick Overview for NT5C2 Protein (AA 1-561) (His tag) (ABIN667570)
Target
See all NT5C2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-561
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Purification tag / Conjugate
- This NT5C2 protein is labelled with His tag.
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Sequence
- MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E
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Characteristics
- NT5C2, 1-561aa, Human, His tag E.coli
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Purification
- > 90% by SDS-PAGE
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5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) protein (GST tag)
NT5C2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
5'-Nucleotidase, Cytosolic II (NT5C2) (AA 182-335) protein (His-IF2DI Tag)NT5C2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
5'-Nucleotidase, Cytosolic II (NT5C2) (AA 1-561) protein (His tag)NT5C2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
5'-Nucleotidase, Cytosolic II (NT5C2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NT5C2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
5'-Nucleotidase, Cytosolic II (NT5C2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NT5C2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 30 % glycerol, 0.1M NaCl,1 mM DTT, 0.1 mM PMSF
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- NT5C2 (5'-Nucleotidase, Cytosolic II (NT5C2))
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Alternative Name
- NT5C2
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Background
- NT5C2, also known as NT5B or PNT5, has an essential role in the maintenance of purine/pyrimidine nucleotides. It contains a phosphotransferase active site that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, it regulates the level of inosine monophosphate (IMP) and guanosine monophosphate (GMP) pools within cells via hydrolysis. Recombinant human NT5C2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 67.1 kDa (581aa)
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NCBI Accession
- NP_036361
Target
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