HMGCL Protein (AA 28-325) (His tag)
Quick Overview for HMGCL Protein (AA 28-325) (His tag) (ABIN667639)
Target
See all HMGCL ProteinsProtein Type
Origin
Source
Application
Purity
-
-
Protein Characteristics
- AA 28-325
-
Purification tag / Conjugate
- This HMGCL protein is labelled with His tag.
-
Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVL KGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISP AKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAALAV HCHDTYGQAL ANTLMALQMG VSVVDSSVAG LGGCPYAQGA SGNLATEDLV YMLEGLGIHT GVNLQKLLEA GNFICQALNR KTSSKVAQAT CKL
-
Characteristics
- HMGCL, 28-325aa , Human, His tag, E.coli
-
Purification
- > 85% by SDS-PAGE
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 1-325) protein (GST tag)
HMGCL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 28-325) protein (His tag)HMGCL Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) protein (Myc-DYKDDDDK Tag)HMGCL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
-
Handling Advice
- Avoid repeated freezing and thawing cycles
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))
-
Alternative Name
- HMGCL
-
Background
- HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA. Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E. coli.
-
Molecular Weight
- 34.2 kDa (323aa)
-
NCBI Accession
- NP_000182
Target
-
