THEM2 Protein (AA 1-140) (His tag)
Quick Overview for THEM2 Protein (AA 1-140) (His tag) (ABIN667825)
Target
See all THEM2 (ACOT13) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-140
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Purification tag / Conjugate
- This THEM2 protein is labelled with His tag.
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Sequence
- MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN
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Characteristics
- ACOT13, 1-140aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Acyl-CoA Thioesterase 13 (ACOT13) (AA 1-140) protein (His tag)
custom-made ACOT13 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Acyl-CoA Thioesterase 13 (ACOT13) (AA 1-140) protein (His tag)custom-made ACOT13 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Acyl-CoA Thioesterase 13 (ACOT13) (AA 1-140) protein (GST tag)ACOT13 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Acyl-CoA Thioesterase 13 (ACOT13) (AA 1-140) protein (His-IF2DI Tag)ACOT13 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Acyl-CoA Thioesterase 13 (ACOT13) protein (Myc-DYKDDDDK Tag)ACOT13 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))
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Alternative Name
- ACOT13
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Background
- Acyl-coenzyme A thioesterase 13, also known ACOT13, belongs to the thioesterase subfamily of esterase family. Highly expressed in kidney with moderate expression in brain, liver and intestines, ACOT13 contains a hotdog-fold and is thought to co-localize with microtubules, possibly playing a role in cellular proliferation events. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Recombinant human ACOT13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 17.1 kDa (160aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_060943
Target
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