RAB13 Protein (AA 1-200) (His tag)
Quick Overview for RAB13 Protein (AA 1-200) (His tag) (ABIN667931)
Target
See all RAB13 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-200
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Purification tag / Conjugate
- This RAB13 protein is labelled with His tag.
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Sequence
- MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC
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Characteristics
- RAB13, 1-200aa, Human, His tag, E.coli
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Purification
- > 85% by SDS-PAGE
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RAB13, Member RAS Oncogene Family (RAB13) (AA 1-203) protein (GST tag)
RAB13 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RAB13, Member RAS Oncogene Family (RAB13) (AA 1-200) protein (His-IF2DI Tag)RAB13 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
RAB13, Member RAS Oncogene Family (RAB13) protein (Myc-DYKDDDDK Tag)RAB13 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 200 mM NaCl, 1 mM EDTA
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- RAB13 (RAB13, Member RAS Oncogene Family (RAB13))
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Alternative Name
- RAB13
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Background
- RAB13 (Ras-related protein Rab-13) belongs to the small GTPase superfamily. Members of the RAB protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. RAB proteins are also an integral part of endocytic pathways. The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs). It is expressed at all stages of preimplantation development. Recombinant human RAB13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 24.7 kDa (220aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002861
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Pathways
- Cell-Cell Junction Organization
Target
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