TNFRSF1B Protein (AA 1-257) (Fc Tag)

Catalog No. ABIN925130

This Recombinant TNFRSF1B protein is produced in HEK-293T Cells.

Quick Overview for TNFRSF1B Protein (AA 1-257) (Fc Tag) (ABIN925130)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Protein Type: Recombinant

Origin: Human

Source: HEK-293T Cells

Application: Apoptosis Blocking (AB)

Purity: > 95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-257

Purification tag / Conjugate: This TNFRSF1B protein is labelled with Fc Tag.

Purpose: Prepare a sterile stock solution of rhTNF-R2:Fc (1 mg/ml in PBS) by dissolving the entire preparation in 50 µl sterile H2O. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.

Specificity: Highly specific for the detection of membrane- bound TNF-alpha.

Characteristics: Host: The extracellular domain of human TNF- R2 (AA 1-257) is fused to the Fc portion of human IgG1.

Application Details

Application Notes: Inhibition of TNF-mediated apoptosis: Exerts inhibitory activity at concentrations higher than 1000 ng/ml. TNF-R2:Fc prefentially interacts with the membrane-bound form of TNF-alpha. Concentrations of rhTNF-R2 required to block killing may vary depending on cell type studied.
Method: Murine WEHI 164 cells (50,000 cells in 100 µl RPMI medium containing 10% fetal calf serum) were plated in a 96 well plate. 100 pg/ml rhsTNF- were incubated with the indicated concentrations of rhTNFR2:Fc for 30 minutes at RT. Then the mixture was added to the WEHI 164 target cells and incubated for at least 12 hr at 37°C. Concentrations of rhsTNF-alpha required to kill cells may vary depending on cell type studied. Concentrations of rhTNFR2:Fc required to block killing may vary depending on cell type studied. Cell viability was determined using a MTT- based cell proliferation assay kit.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS

Storage: -20 °C

Target Details

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: TNF-R2

Background: TNF-Receptors (TNF-R) are implicated in inflammatory processes and are the prototype members of the TNF receptor family. There are two distinct TNF-R, i. e. TNF-R1 and TNF-R2, that interact with the ligand TNF-. Whereas both receptors activate NF-B and JNK, TNF-R1 also signals cell death. Activated TNF-R1 contains a death domain in its cytoplasmic region that recruits the adaptor proteins TRADD, RIP, FADD, and TRAF2. TNF-R2 recruits TRAF proteins. Whereas membrane-bound and processed, soluble TNF- bind to TNF-R1, only the membrane-bound form appears to interact with TNF-R2.

Molecular Weight: 80 kDa (chimeric protein)

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway