APOA1 Protein
Quick Overview for APOA1 Protein (ABIN935203)
Target
See all APOA1 ProteinsProtein Type
Origin
Source
Purity
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Sequence
- MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ
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Characteristics
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Purified recombinant Human ApoA-I protein
Expression System: E.coli -
Endotoxin Level
- < 0.1 ng per μg (1 EU/μg).
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Apolipoprotein A-I (APOA1) (AA 25-267) (Active) protein (hFc Tag)
APOA1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Apolipoprotein A-I (APOA1) (AA 25-264) (Active) protein (hFc Tag)APOA1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Apolipoprotein A-I (APOA1) (AA 1-267) protein (GST tag)APOA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Apolipoprotein A-I (APOA1) (AA 25-267) protein (His tag)APOA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 1.0 mg/mL.
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Buffer
- Lyophilized from 5 mM Na3PO4, pH 7.5.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- APOA1 (Apolipoprotein A-I (APOA1))
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Alternative Name
- ApoA-I
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Background
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ApoA-I is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis.
Alternative Names: Apolipoprotein A-I protein, ApoA I protein, ApoA1 protein, Apo AI protein -
Molecular Weight
- 28.2 kDa
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Pathways
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
Target
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