anti-AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B) Antibodies

This locus encodes an AT-rich DNA interacting domain-containing protein. Additionally we are shipping and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ARID1B 239985  
ARID1B 282546  
ARID1B 57492 Q8NFD5
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Top anti-ARID1B Antibodies at antibodies-online.com

Showing 10 out of 46 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of BAF250B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of BAF250B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, WB Western Blot: ARID1B Antibody [ABIN252823] - Detection of Human ARID1B by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARID1B antibody ABIN252823 used for IP at 3 mcg/mg lysate. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody ABIN252822, which recognizes an upstream epitope.Detection: Chemiluminescence with an exposure time of 30 seconds. Immunohistochemistry-Paraffin: ARID1B Antibody [ABIN2528235] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-ARID1B used at a dilution of 1:250. 100 μL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated IF, ELISA, WB Western blot analysis of extracts from LOVO cells, using BAF250B Antibody. The lane on the right is treated with the synthesized peptide. Immunofluorescence analysis of HuvEc cells, using BAF250B Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Mouse Un-conjugated IF, IHC, WB Immunofluorescence of monoclonal antibody to ARID1B on HeLa cell (antibody concentration 10 µg/mL). ARID1B monoclonal antibody (M02), clone 2F2 Western Blot analysis of ARID1B expression in Hela NE. 100 μg Log in to see 8 to 11 Days
$461.00
Details
Human Mouse Un-conjugated ELISA, IF, IHC, WB Immunofluorescence of monoclonal antibody to ARID1B on HeLa cell (antibody concentration 10 µg/mL). ARID1B monoclonal antibody (M01), clone 2D2 Western Blot analysis of ARID1B expression in HeLa NE. 100 μg Log in to see 8 to 11 Days
$461.00
Details
Human Mouse Un-conjugated IF, IHC (p), ELISA, WB Immunoperoxidase of monoclonal antibody to ARID1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Immunofluorescence of monoclonal antibody to ARID1B on HeLa cell. [antibody concentration 10 ug/ml] 100 μg Log in to see 11 to 12 Days
$440.00
Details
Human Rabbit Un-conjugated ELISA, IF, IHC, WB 100 μL Log in to see 16 Days
$181.73
Details
Human Rabbit Un-conjugated IHC, IP, WB Western Blot and Immunoprecipitation: ARID1B Antibody [ABIN252822] - Detection of Human ARID1B by Western Blot and Immunoprecipitation.>/b> Samples: Whole cell lysate (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARID1B antibody ABIN252822 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-47485, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B). Western Blot: ARID1B Antibody - Detection of Human ARID1B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. 0.1 mL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, WB ABIN6274434 staining LOVO cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibody. Western blot analysis of extracts from LOVO cells, using BAF250B antibody. 100 μL Log in to see 11 to 12 Days
$390.77
Details
Human Mouse Un-conjugated ELISA, IF, IHC, IHC (p), WB 100 μg Log in to see 7 to 9 Days
$537.17
Details

Top referenced anti-ARID1B Antibodies

  1. Human Monoclonal ARID1B Primary Antibody for IF, IHC (p) - ABIN566118 : Coatham, Li, Karnezis, Hoang, Tessier-Cloutier, Meng, Soslow, Blake Gilks, Huntsman, Stewart, Postovit, Köbel, Lee: Concurrent ARID1A and ARID1B inactivation in endometrial and ovarian dedifferentiated carcinomas. in Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 (PubMed)
    Show all 2 Pubmed References

  2. Human Monoclonal ARID1B Primary Antibody for IF, IHC (p) - ABIN528194 : Flores-Alcantar, Gonzalez-Sandoval, Escalante-Alcalde, Lomelí: Dynamics of expression of ARID1A and ARID1B subunits in mouse embryos and in cells during the cell cycle. in Cell and tissue research 2011 (PubMed)

  3. Human Polyclonal ARID1B Primary Antibody for IHC, IP - ABIN252822 : Sim, White, Fitzpatrick, Wilson, Gillies, Pope, Mountford, Torring, McKee, Vulto-van Silfhout, Jhangiani, Muzny, Leventer, Delatycki, Amor, Lockhart: Expanding the phenotypic spectrum of ARID1B-mediated disorders and identification of altered cell-cycle dynamics due to ARID1B haploinsufficiency. in Orphanet journal of rare diseases 2014 (PubMed)

  4. Human Monoclonal ARID1B Primary Antibody for ELISA, IF - ABIN395499 : Watanabe, Ui, Kanno, Ogiwara, Nagase, Kohno, Yasui: SWI/SNF factors required for cellular resistance to DNA damage include ARID1A and ARID1B and show interdependent protein stability. in Cancer research 2014 (PubMed)

More Antibodies against ARID1B Interaction Partners

Mouse (Murine) AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B) interaction partners

  1. Genome-wide gene expression patterns in Arid1b hKO mice were similar to those in Autism spectrum disorder (ASD (show GUSB Antibodies))patients and Chd8 (show CHD8 Antibodies)-haploinsufficient mice, an ASD (show GUSB Antibodies) model, and to developmental changes in gene expression in fast-spiking cells in the mouse brain. Our results suggest that Arid1b haploinsufficiency causes ASD (show GUSB Antibodies)-like phenotypes in mice.

  2. In the brain, Arid1b haploinsufficiency resulted in changes in the expression of SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies)-regulated genes implicated in neuropsychiatric disorders.

  3. STAT3 (show STAT3 Antibodies)/Arid1b/beta-catenin (show CTNNB1 Antibodies) pathway is driving neurofibroma initiation.

  4. The patterns of expression of ARID1A (show ARID1A Antibodies) and ARID1B genes through various mouse embryonic stages, was examined.

  5. These data suggest that the BAF250B-associated SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies) is essential for mouse embryonic stem cells to maintain their normal proliferation and pluripotency.

  6. A subset of mammalian SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies) complexes, specifically containing ARID1B, is required for efficient cell cycle re-entry and for association of activation factors with the c-myc (show MYC Antibodies) promoter.

Human AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B) interaction partners

  1. We report on a 4-year-old male child who was found to be heterozygous for ARID1B de novo pathogenic NM_020732.3:c.3323_3324delAA; p.(Lys1108Argfs) mutation. The coding region (+/-5 bp) of the SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies) complex genes showed a de novo heterozygous sequence change in the ARID1B gene: NM_020732.3:c.3323_3324delAA; p.(Lys1108Argfs). The variant was confirmed by Sanger sequencing and was not detected in the parental samples.

  2. the correlation between the loss of ARID1A (show ARID1A Antibodies) immunoreactivity and reduced ARID1B levels indicates that ARID1B could be an attractive target for anti-cancer therapy.

  3. findings establish mutations in ARID1B as the underlying genetic defect in the HHID syndrome in two of three patients.

  4. The authors find that ARID1A (show ARID1A Antibodies) has a dominant role in maintaining chromatin accessibility at enhancers, while the contribution of ARID1B is evident only in the context of ARID1A (show ARID1A Antibodies) mutation.

  5. we identified a subgroup of neuroblastoma (show ARHGEF16 Antibodies) with ARID1B mutation shows an aggressive behavior. These findings may provide a new biomarker to identify another subgroup of neuroblastoma (show ARHGEF16 Antibodies) with high-risk features.

  6. these results highlighted the significant genetic contribution of the ARID1B variant, rs73013281, to susceptibility for HCC (show FAM126A Antibodies), especially in interaction with physical activity.

  7. we identified concurrent ARID1A (show ARID1A Antibodies) and ARID1B inactivating mutations with consequent loss of protein expression in the undifferentiated component of approximately one-quarter of dedifferentiated endometrial and ovarian carcinomas

  8. Of the 34 undifferentiated endometrial carcinomas examined, 17 (50%) exhibited SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies) complex inactivation, with 11 tumors showing complete loss of both ARID1A (show ARID1A Antibodies) and ARID1B, 5 showing complete loss of BRG1 (show SMARCA4 Antibodies) and 1 showing complete loss of INI1 (show SMARCB1 Antibodies). Ten of the remaining 17 undifferentiated carcinomas showed the following alterations: 5 tumors (15%) showed loss of ARID1A (show ARID1A Antibodies) only with intact ARID1B, BRG1 (show SMARCA4 Antibodies), and INI1 (show SMARCB1 Antibodies) expression.

  9. We report here the clinical, genetic, and proteomic phenotypes of an individual with a unique apparent de novo mutation of ARID1B due to an intragenic duplication

  10. HSCR (show EDNRB Antibodies) was observed in a patient with a truncating mutation in ARID1B, further expanding the phenotypic spectrum of Coffin-Siris syndrome. This suggests that the BAF (show BANF1 Antibodies) complex does not only play a role in the enteric system of Drosophila, but also in humans.

ARID1B Antigen Profile

Protein Summary

This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene names and symbols associated with ARID1B

  • AT rich interactive domain 1B (SWI1-like) (arid1b) antibody
  • AT rich interactive domain 1B (SWI-like) (Arid1b) antibody
  • AT-rich interaction domain 1B (Arid1b) antibody
  • AT-rich interaction domain 1B (ARID1B) antibody
  • 6A3-5 antibody
  • 8030481M12 antibody
  • 9330189K18Rik antibody
  • AI836955 antibody
  • Ardi1b antibody
  • B230217J03Rik antibody
  • BAF250B antibody
  • BRIGHT antibody
  • DAN15 antibody
  • ELD/OSA1 antibody
  • fd07d11 antibody
  • mKIAA1235 antibody
  • MRD12 antibody
  • OSA2 antibody
  • P250R antibody
  • si:dkey-60a16.3 antibody
  • smcf-1 antibody
  • Smcf1 antibody
  • wu:fd07d11 antibody

Protein level used designations for ARID1B

AT rich interactive domain 1B (Swi1 like) , AT-rich interactive domain-containing protein 1B , transcription factor 1 , ARID domain-containing protein 1B , BRG1-associated factor 250b , BRG1-binding protein ELD/OSA1 , ELD (eyelid)/OSA protein

GENE ID SPECIES
569589 Danio rerio
239985 Mus musculus
282546 Rattus norvegicus
57492 Homo sapiens
421650 Gallus gallus
484047 Canis lupus familiaris
505229 Bos taurus
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