anti-Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) Antibodies

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B\; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008].. Additionally we are shipping CHAF1A Proteins (4) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CHAF1A 363333  
CHAF1A 10036 Q13111
CHAF1A 27221 Q9QWF0
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Top anti-CHAF1A Antibodies at antibodies-online.com

Showing 10 out of 57 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of extracts from Jurkat cells, using CAF1B Antibody. The lane on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ELISA, WB 100 μL Available
$363.46
Details
Human Mouse Un-conjugated IF, IHC (p), WB 0.1 mg 6 to 8 Days
$357.50
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, WB ABIN6274332 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibody. Western blot analysis of extracts from various samples, using CAF1B Antibody. Lane 1: Mouse cancer treated with blocking peptide. Lane 2: Mouse cancer; Lane 3: mouse brain; 100 μL 11 to 12 Days
$390.77
Details
Human Rabbit Un-conjugated ELISA, WB   100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated WB Western blot analysis of extracts of various cell lines, using CHAF1A antibody. 200 μL 12 to 14 Days
$438.90
Details
Human Mouse Un-conjugated DB   100 μg 11 to 14 Days
$837.83
Details
Human Mouse Un-conjugated WB   100 μg 11 to 14 Days
$815.83
Details
Human Mouse Un-conjugated ELISA, WB CHAF1A monoclonal antibody (M02), clone 1C2 Western Blot analysis of CHAF1A expression in Hela S3 NE . Detection limit for recombinant GST tagged CHAF1A is 0.1 ng/ml as a capture antibody. 100 μg 11 to 12 Days
$440.00
Details
Human Hamster Un-conjugated ICC, IF, IP, WB   100 μg 1 to 2 Days
$507.50
Details

Top referenced anti-CHAF1A Antibodies

  1. Human Monoclonal CHAF1A Primary Antibody for ICC, IF - ABIN153013 : Smith, Stillman: Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro. in The Journal of biological chemistry 1991 (PubMed)
    Show all 11 Pubmed References

  2. Monoclonal CHAF1A Primary Antibody for IF, IP - ABIN534079 : Shibahara, Stillman: Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. in Cell 1999 (PubMed)
    Show all 5 Pubmed References

  3. Human Monoclonal CHAF1A Primary Antibody for ICC, IF - ABIN2668347 : Hoek, Stillman: Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo. in Proceedings of the National Academy of Sciences of the United States of America 2003 (PubMed)
    Show all 3 Pubmed References

  4. Human Monoclonal CHAF1A Primary Antibody for ELISA, WB - ABIN564324 : Doe, Nozawa, Hiruma, Yamada, Matsuki, Nakano, Ogasawara, Nakano, Ikeda, Ikegami, Fujishiro, Kawasaki, Ikeda, Amano, Morimoto, Ogawa, Takamori, Sekigawa, Takasaki: Antibody against chromatin assembly factor-1 is a novel autoantibody specifically recognized in systemic lupus erythematosus. in Lupus 2014 (PubMed)

  5. Human Polyclonal CHAF1A Primary Antibody for IHC (p), IP - ABIN261685 : Li, Xu, Chang, Zheng, Shen, Liu: SUMO2 conjugation of PCNA facilitates chromatin remodeling to resolve transcription-replication conflicts. in Nature communications 2018 (PubMed)

More Antibodies against CHAF1A Interaction Partners

Human Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) interaction partners

  1. These and other approaches identified the Nucleosome Remodeling Deacetylase (NuRD) and Chromatin Assembly Factor 1 (CAF-1) complexes as necessary for DUX4 repression in human skeletal muscle cells and induced pluripotent stem (iPS) cells.Furthermore, DUX4-induced expression of MBD3L proteins partly relieved this repression in FSHD muscle cells.

  2. this study identified a novel oncogenic role of CHAF1A in GC. The upregulation of CHAF1A can promote GC cell growth and predict poor prognosis in GC patients.

  3. CHAF1A and PCNA are highly expressed in cervical squamous cell carcinoma and associated with the malignancy

  4. Recent work has identified the chromatin assembly factor complex CAF-1 as a potent barrier to cellular reprogramming. In addition, CAF-1 has been implicated in the reversion of pluripotent cells to a totipotent-like state and in various lineage conversion paradigms, suggesting that modulation of CAF-1 levels may endow cells with a developmentally more plastic state.

  5. Chromatin reassembly during double-strand break repair was dependent on the HIRA histone chaperone that is specific to the replication-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent canonical histones H3.1/H3.2.

  6. Data show that the high expression of chromatin assembly factor 1, subunit A (p150) (CHAF1A) promotes cell proliferation and inhibits cell apoptosis, suggesting that CHAF1A may be developed as a prognosis biomarker and potential therapeutic target of epithelial ovarian cancer (EOC).

  7. CHAF1A inhibits NEIL1 initiated repair in vitro Subsequent restoration of the chaperone-BER complex in cell, presumably after completion of repair, suggests that histone chaperones sequester the repair complex for oxidized bases in non-replicating chromatin, and allow repair when oxidized bases are induced in the genome.

  8. Loss of CAF1 is associated with increased motility and invasive phenotypes seen in transformed cells.

  9. These data suggest that CAF-1- and ASF1A-H3-H4-dependent deposition of the histone (H3-H4)2 tetramers is compatible with MMR and protects the discontinuous daughter strand from unnecessary degradation by MMR machinery.

  10. Taken together, these results show that CHAF1A contributes to the proliferation of glioblastoma cells and may be developed as a de novo drug target and prognosis biomarker of glioblastoma.

  11. Novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus.

  12. The frequency of positive CHAF1A staining in primary tumor mucosa (45.8% of 203 samples) was significantly elevated compared to normal mucosa (18.7% of samples). Increased expression was associated with cancer stage, tumor invasion and histological grade.

  13. Histone chaperone CHAF1A inhibits differentiation and promotes aggressive neuroblastoma.

  14. CAF1 was hijacked by IE2 to facilitate the replication of the HCMV genome, suggesting chromatin assembly plays an important role in herpesviral DNA synthesis, but also provide a model of the virus-induced chromatin instability through CAF1.

  15. CAF-I-dependent control of degradation of the discontinuous strands during mismatch repair.

  16. these findings suggest an expanded role of p150 as a SUMO2/3-interacting factor, and raise the intriguing possibility that p150 plays a role in promoting delivery of SUMO2/3 or SUMO2/3-modified proteins (or both) on chromatin fibers during replication.

  17. p150 was efficiently phosphorylated by Cdc7-Dbf4 kinase.

  18. ACF generates the characteristic 50- to 60-base-pair internucleosomal spacing in silent chromatin by kinetically discriminating against shorter linker DNAs

  19. rs9352 of CHAF1A was repeatedly includes (78% of the 200 bootstrap samples) in the best set of SNPs. CHAF1A may contribute to gliomagenesis.

  20. p150 directly activates transcription, independently of its histone deposition function.

Mouse (Murine) Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) interaction partners

  1. CAF-1 is an essential guardian of the genome in preimplantation mouse embryos.

  2. the histone chaperone CAF-1 to be a novel regulator of somatic cell identity during transcription-factor-induced cell-fate transitions and provide a potential strategy to modulate cellular plasticity in a regenerative setting

  3. Findings suggest that p150 may link the DNA-bound Gfi1 repressor complex to histones enabling modifications required for transcriptional silencing.

  4. This recruitment is strictly dependent on p150CAF-1, the largest subunit of chromatin assembly factor 1 (CAF-1), and its ability to interact with HP1alpha.

  5. during DNA replication, the interaction of HP1 with p150CAF-1 is essential to promote delivery of HP1 molecules to heterochromatic sites

  6. findings suggest that CAF-1 is required for the maturation of heterochromatin during preimplantation development and that CAF-1 is specifically required for heterochromatin organization in pluripotent embryonic cells

  7. Data show that human CAF-1 (p150) contains two PCNA interaction peptides, and suggest that these peptides are part of the mechanism that enables CAF-1 to function behind replication forks without interfering with other PCNA-mediated processes.

CHAF1A Antigen Profile

Protein Summary

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B\; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995

Gene names and symbols associated with CHAF1A

  • chromatin assembly factor 1 subunit A (Chaf1a) antibody
  • chromatin assembly factor 1 subunit A (CHAF1A) antibody
  • chromatin assembly factor 1 subunit A (LOC100447657) antibody
  • chromatin assembly factor 1, subunit A (p150) (Chaf1a) antibody
  • chromatin assembly factor 1, subunit A (p150) (chaf1a) antibody
  • chromatin assembly factor 1 subunit A S homeolog (chaf1a.S) antibody
  • AL023013 antibody
  • AL024058 antibody
  • Cac1p antibody
  • CAF-1 antibody
  • CAF-1P150 antibody
  • CAF-I-p150 antibody
  • CAF-Ip150 antibody
  • CAF1 antibody
  • CAF1B antibody
  • CAF1P150 antibody
  • caip150 antibody
  • CHAF1A antibody
  • chaf1a-b antibody
  • P150 antibody
  • wu:fd14g10 antibody
  • xp150 antibody
  • zgc:153930 antibody

Protein level used designations for CHAF1A

chromatin assembly factor 1, subunit A (p150) , chromatin assembly factor 1 subunit A-like , CAF-1 subunit A , CAF-I 150 kDa subunit , CAF-I p150 , chromatin assembly factor 1 subunit A , chromatin assembly factor I (150 kDa) , chromatin assembly factor I p150 subunit , hp150 , CAF-Ip150 , chromatin assembly factor-1p150 , Chromatin assembly factor I p150 subunit , CAF-I 150 kDa subunit B , CAF-I p150-B , CAF-Ip150-B , Chromatin assembly factor I p150 subunit B , chromatin assembly factor 1 subunit A-B

GENE ID SPECIES
363333 Rattus norvegicus
455603 Pan troglodytes
485038 Canis lupus familiaris
100231993 Taeniopygia guttata
100447657 Pongo abelii
100590477 Nomascus leucogenys
10036 Homo sapiens
27221 Mus musculus
504535 Bos taurus
770217 Gallus gallus
563212 Danio rerio
733264 Xenopus laevis
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