anti-Diaphanous Homolog 3 (Drosophila) (DIAPH3) Antibodies

DIAPH3 encodes a member of the diaphanous subfamily of the formin family. Additionally we are shipping DIAPH3 Proteins (4) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
DIAPH3 290396  
DIAPH3 56419 Q9Z207
DIAPH3 81624 Q9NSV4
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Top anti-DIAPH3 Antibodies at antibodies-online.com

Showing 10 out of 77 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Human Goat Un-conjugated ELISA   100 μg 6 to 7 Days
$291.53
Details
Human Rabbit Un-conjugated EIA, WB Western blot analysis of DIAPH3 Antibody 0.4 mL 6 to 8 Days
$484.00
Details
Human Rabbit Un-conjugated IHC (p) IHC-P Image DIAPH3 antibody [C1C3] detects DIAPH3 protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. DIAPH3 antibody [C1C3] , dilution: 1:100. 100 μL 3 to 4 Days
$466.18
Details
Human Rabbit Un-conjugated IHC, IHC (p), WB Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-DIAPH3 / MDIA2 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 50 μL 11 to 14 Days
$484.00
Details
Human Rabbit Un-conjugated IHC, WB ABIN6273041 at 1/100 staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. Western blot analysis of extracts of HEK-293 cells, using DIAPH3 antibody. 100 μL 11 to 12 Days
$390.77
Details
Human Mouse Un-conjugated IF, ELISA, WB Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody. Western Blot analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate. 100 μg 11 to 12 Days
$440.00
Details
Human Rabbit Un-conjugated WB Western blot analysis in NCI-H460 cell line lysates (35ug/lane). 400 μL 2 to 3 Days
$515.63
Details
Human Rabbit Un-conjugated IP, WB Immunoprecipitation of DIAPH3 transfected lysate using anti-DIAPH3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead , and immunoblotted with DIAPH3 purified MaxPab mouse polyclonal antibody (B01P) . Western Blot analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 MaxPab polyclonal antibody.Lane 1: DIAPH3 transfected lysate(98.1 KDa).Lane 2: Non-transfected lysate. 100 μL 11 to 12 Days
$425.71
Details
Human Mouse Un-conjugated WB Western Blot analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 MaxPab polyclonal antibody.Lane 1: DIAPH3 transfected lysate(98.10 KDa).Lane 2: Non-transfected lysate. 50 μL 11 to 12 Days
$440.00
Details
Human Rabbit Un-conjugated WB Western blot analysis of extracts of NCI-H460 cells, using DIAPH3 antibody (ABIN5972907). 100 μL 11 to 16 Days
$426.40
Details

Top referenced anti-DIAPH3 Antibodies

  1. Human Polyclonal DIAPH3 Primary Antibody for ELISA - ABIN449532 : Yang, Czech, Gerboth, Kojima, Scita, Svitkina: Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells. in PLoS biology 2007 (PubMed)

  2. Human Polyclonal DIAPH3 Primary Antibody for ELISA, WB - ABIN566471 : Kyrkou, Soufi, Bahtz, Ferguson, Bai, Parton, Hoffmann, Zerial, Fotsis, Murphy: RhoD participates in the regulation of cell-cycle progression and centrosome duplication. in Oncogene 2013 (PubMed)

More Antibodies against DIAPH3 Interaction Partners

Mouse (Murine) Diaphanous Homolog 3 (Drosophila) (DIAPH3) interaction partners

  1. neuronal drebrin A directly interacts with mDia2 formin.

  2. The authors report that formins mDia1 and mDia2 dissociate faster under higher ionic strength and when actin concentration is increased. Profilin, known to increase the elongation rate of formin-associated filaments, surprisingly decreases the formin dissociation rate, by bringing formin FH1 domains in transient contact with the barbed end.

  3. These data identify Diaph3 as a major guard of cortical progenitors, unravel novel functions of Diaphanous formins and add insights into the pathobiology of microcephaly.

  4. mDia2 is regulated through acetylation and deacetylation at lysine 970 in the formin homology 2 domain

  5. findings add more complexity to the mDia2 activity cycle by showing that the open conformation may control actin dynamics also through actin-independent regulation of the proteasome

  6. Ineffective erythropoiesis caused by binucleated late-stage erythroblasts in mDia2 hematopoietic specific knockout mice.

  7. the interactome of auto-inhibited and constitutively active mDia2, is reported.

  8. mDia2-deficient erythroid cells differentiate normally, though in a delayed manner, but exhibit cytokinesis failure with decreased accumulation of F-actin in the cleavage furrow during late differentiation from proerythroblasts

  9. Diaphanous homolog 3 (Diap3) overexpression causes progressive hearing loss and inner hair cell defects in a transgenic mouse model of human deafness.

  10. Dia1 and Dia2 are dynamic components of meiotic spindle and pole complex during meiotic maturation of oocytes.

  11. Microtubules strongly inhibit actin polymerization by mDia2.

  12. Results indicate that ROCK-dependent phosphorylation of the mDia2 DAD is an important determinant of mDia2 activity and that this signalling mechanism affects actin polymerization and smooth muscle cell-specific gene expression.

  13. Results show that targeting of mDia2 to the cleavage furrow requires not only its binding to RhoA but also its diaphanous-inhibitory domain, and identify anillin as a novel mDia2 interaction partner.

  14. Introduction of the mDia2 inhibitor DIP/WISH/SPIN90 into cells triggers non-apoptotic membrane blebbing. The results infer a role for mDia2 in the maintenance of actin networks supporting the cell cortex.

  15. the bundling activities of FRL1 and mDia2, while producing phenotypically similar bundles, differ in mechanistic detail

  16. the DD/CC region is required for the Rho-independent membrane targeting of the isolated N terminus of mDia1 and mDia2

  17. Dia2 plays an important role in formation of lamellipodia.

  18. Rac GTPases and their effector mDia2 have important roles in enucleation of mammalian erythroblasts

  19. These results demonstrate that mDia2 is essential in mammalian cell cytokinesis and that mDia2-induced F-actin forms a scaffold for the contractile ring and maintains its position in the middle of a dividing cell.

  20. analysis of interactions between mDia isoforms 1, 2, and 3 and RhoA, Rac1, and Cdc42

Human Diaphanous Homolog 3 (Drosophila) (DIAPH3) interaction partners

  1. through profilin-1 binding, CLIC4 functions in a RhoA-mDia2-regulated signaling network to integrate cortical actin assembly and membrane protrusion

  2. These data identify Diaph3 as a major guard of cortical progenitors, unravel novel functions of Diaphanous formins and add insights into the pathobiology of microcephaly.

  3. study demonstrated the oncogenic activity of DIAPH3 in the progression of hepatocellular carcinoma and suggested that PDIAPH3 might be a therapeutic target.

  4. Members of 3 generations of a family had dominant auditory neuropathy associated with a c.-172G>A point mutation in DIAPH3.

  5. DIAPH3 overexpression inhibits the migration and invasion of Triple-negative breast cancer by inhibiting RhoA-GTP expression.

  6. mDia2 and CXCR4 associate in blebs upon CXCL12 stimulation. Both CXCR4 and RhoA are required for CXCL12-induced blebbing

  7. we use a simple cellular system to examine fundamental features of formin-mediated filopodial assembly, using constitutively active constructs of the formins mDia2 and FMNL3

  8. results suggest that inhibition of MT stability arising from DIAPH3 downregulation enhances susceptibility to MT poisons, and that the DIAPH3 network potentially reports taxane sensitivity in human tumors

  9. Dia1, Dia2, and Dia3 are involved in ErbB2-dependent capture of microtubules at the cell leading edge and ErbB2-driven guided migration.

  10. An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.

  11. TGF-beta promotes association of mDia2 with actin stress fibers, which further drives stress fiber formation and myofibroblast differentiation

  12. In human tumours, DIAPH3 down-regulation was associated with aggressive or metastatic disease.

  13. isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

  14. the amino terminus of mDia2 serves as a coincidence detection module, directing mDia2 to the plasma membrane through interactions with phospholipids and activated Rif

  15. These findings reveal a key role for mDia3 and its regulation by Aurora B phosphorylation in achieving proper stable kinetochore microtubule attachment.

  16. These studies further suggest that Rho GTPases significantly influence the activity of mDia family formins in driving cellular membrane remodeling through the regulation of actin dynamics.

  17. DIP-induced membrane blebbing was dependent on Diaphanous 2 but did not require the activities of either Diaphanous 1 or Actin-Related Protein 2-3 Complex.

  18. Data show that Dia2 localizes in the lamella of migrating epithelial cells, where it is involved in the formation of a stable pool of cortical actin and in maintenance of polymerization-competent free filament barbed ends at focal adhesions.

  19. Results suggest that WAVE and the Arp2/3 complex jointly orchestrate different types of actin-based plasma membrane protrusions by promoting ruffling and inhibiting mDia2-induced filopodia.

  20. active Drf3 lacking the C-terminal regulatory region (Drf3DeltaDAD) induced the formation of filopodia and accumulated at their tips.

DIAPH3 Antigen Profile

Protein Summary

This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with DIAPH3

  • diaphanous-related formin 3 (Diaph3) antibody
  • diaphanous related formin 3 (DIAPH3) antibody
  • diaphanous homolog 3 (Drosophila) (DIAPH3) antibody
  • diaphanous related formin 3 (Diaph3) antibody
  • 4930417P13Rik antibody
  • AN antibody
  • AUNA1 antibody
  • DIA2 antibody
  • diap3 antibody
  • Diaph3 antibody
  • DRF3 antibody
  • mDia2 antibody
  • mKIAA4117 antibody
  • NSDAN antibody
  • p134MDia2 antibody

Protein level used designations for DIAPH3

diaphanous homolog 3 , protein diaphanous homolog 3 , diaphanous homolog 3 (Drosophila) , protein diaphanous homolog 3-like , diaphanous-related formin , diaphanous-related formin-3

GENE ID SPECIES
290396 Rattus norvegicus
418824 Gallus gallus
100174272 Pongo abelii
485474 Canis lupus familiaris
525628 Bos taurus
452752 Pan troglodytes
100051814 Equus caballus
100011744 Monodelphis domestica
100475459 Ailuropoda melanoleuca
100517419 Sus scrofa
56419 Mus musculus
81624 Homo sapiens
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