anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) Antibodies

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. Additionally we are shipping GCLM Kits (48) and GCLM Proteins (13) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
GCLM 29739 P48508
GCLM 2730 P48507
GCLM 14630 O09172
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Top anti-GCLM Antibodies at antibodies-online.com

Showing 10 out of 85 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated IHC, WB 100 μL Log in to see 2 to 3 Days
$319.00
Details
Human Rabbit Un-conjugated FACS, IF, IHC (p), WB Western blot analysis of GCLM Antibody (C-term) (ABIN653046) in 293 cell line and mouse kidney tissue lysates (35 µg/lane). GCLM (arrow) was detected using the purified polyclonal antibody. GCLM Antibody (C-term)(ABIN653046) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining 400 μL Log in to see 10 to 11 Days
$385.00
Details
Cow Rabbit Un-conjugated IHC, WB Immunohistochemical analysis of GCLM staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of GCLM expression in SHSY5Y (A), HeLa (B), 3T3 (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated ICC, IF, IHC (p), IHC, WB Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP1-33405] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution. Western Blot: GCLM Antibody [NBP1-33405] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000. 100 μL Log in to see 8 to 11 Days
$516.98
Details
Human Rabbit Un-conjugated ICC, IF, WB Western Blot: GCLM Antibody [NBP2-16624] - Sample (30 ug of whole cell lysate) A: C8D30 12% SDS PAGE gel, diluted at 1:1000. Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP2-16624] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution. 0.1 mL Log in to see 8 to 11 Days
$516.98
Details
Human Rabbit Un-conjugated IF, IHC, WB 100 μL Log in to see 16 Days
$366.77
Details
Bat Rabbit Un-conjugated IHC, IHC (p), WB Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-GCLM antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms ... 50 μg Log in to see 11 to 14 Days
$727.83
Details
Human Rabbit Un-conjugated ELISA, IHC, WB Western blot analysis of MCF7 cell lysate, using GCLM Antibody. The lane on the left is treated with the antigen-specific peptide. ABIN6277512 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary 100 μL Log in to see 11 to 12 Days
$390.77
Details
Human Mouse Un-conjugated ELISA, WB Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (55.88 KDa) . Dilution: 1:500~1000 100 μg Log in to see 8 to 11 Days
$527.50
Details
Human Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Human A431 cell lysate; Primary Ab: 5µg/ml Rabbit Anti-Human GCLM Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Figure. Western Blot; Sample: Recombinant GCLM, Human. 100 μg Log in to see 13 to 16 Days
$332.00
Details

Top referenced anti-GCLM Antibodies

  1. Human Monoclonal GCLM Primary Antibody for ELISA, WB - ABIN516058 : Balamurugan, Akhov, Selvaraj, Pugazhenthi: Induction of antioxidant enzymes by curcumin and its analogues in human islets: implications in transplantation. in Pancreas 2009 (PubMed)
    Show all 4 Pubmed References

  2. Cow (Bovine) Polyclonal GCLM Primary Antibody for IHC, WB - ABIN2785805 : Jönsson, Jönsson, Axmon, Littorin, Broberg: Influence of glutathione-related genes on symptoms and immunologic markers among vulcanization workers in the southern Sweden rubber industries. in International archives of occupational and environmental health 2008 (PubMed)
    Show all 3 Pubmed References

  3. Human Polyclonal GCLM Primary Antibody for ELISA, IHC - ABIN6261915 : Zou, Wang, Peng, Wei: Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells. in Oxidative medicine and cellular longevity 2017 (PubMed)
    Show all 2 Pubmed References

  4. Human Polyclonal GCLM Primary Antibody for FACS, IF - ABIN653046 : Moyer, Sun, Batzler, Li, Schaid, Yang, Weinshilboum: Glutathione pathway genetic polymorphisms and lung cancer survival after platinum-based chemotherapy. in Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2010 (PubMed)
    Show all 2 Pubmed References

  5. Human Polyclonal GCLM Primary Antibody for ICC, IF - ABIN443275 : Lu, Samanta, Xiang, Zhang, Hu, Chen, Bullen, Semenza: Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. in Proceedings of the National Academy of Sciences of the United States of America 2015 (PubMed)

More Antibodies against GCLM Interaction Partners

Human Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) interaction partners

  1. Glutathione biosynthesis during the lipopolysaccharide-induced inflammatory response in THP-1 macrophages is tightly and differentially regulated via GCLC and GCLM subunits of glutamate cysteine ligase.

  2. Two promoter polymorphisms of GCLM (-588C/T) and GCLC (-128T/C) are associated with an increased risk of ischemic heart disease in Kazakhstan population.

  3. High GCLM expression is associated with chemotherapy resistance in breast cancer.

  4. Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1beta regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2

  5. Data suggest gene expression in vascular endothelium is altered by dietary factors; aged garlic extract induces expression of HMOX1 (heme oxygenase-1) and GCLM via activation of NRF2-ARE (nuclear factor erythroid 2-antioxidant response element) signaling.

  6. miR-433 targets both catalytic (GCLc) and regulatory (GCLm) subunits of GCL.

  7. Data suggest expression of hepatocyte GCLC and GCLM can be regulated by dietary component; alpha-lipoic acid, a vitamin B complex nutrient, protects against oxidative stress/cytotoxicity induced by cadmium via restoration of GCLC and GCLM expression.

  8. miR-320a has a role in modulating the induction of HO-1, GCLM and OKL38 by oxidized phospholipids in endothelial cells

  9. This study demonstrated that Tuberous sclerosis complex neuropathology requires glutamate-cysteine ligase.

  10. Knockdown of glutamate cysteine ligase catalytic subunit by siRNA causes the gold nanoparticles-induced cytotoxicity in lung cancer cells.

  11. Upregulation of both GCLC and GLCM mRNA levels in response to cysteine deprivation was dependent on new protein synthesis.

  12. Ten of the 17 maternal SNPs and 2 of the 17 fetal SNPs were found within the GCLM gene conotruncal heart defect cases.

  13. TP is expressed at high levels in human GBM tumors and shRNA knockdown of TP in U87 GBM cells results in a significant increase in cellular GCL enzymatic activity

  14. SNPs not associated with self-reported depression

  15. When eicosapentaenoic+docosahexaenoic acid or erythrocyte-mercury were divided into tertiles, GCLM-588 TT genotype individuals showed a lower risk than CC genotype; however, there were few TT carriers and the results were not statistically significant.

  16. GCLM is a functional target gene of the BACH1 transcription factor according to ChIP-seq and knockdown analysis in HEK 293 cells.

  17. Posttranslational modification and regulation of glutamate-cysteine ligase by the alpha,beta-unsaturated aldehyde 4-hydroxy-2-nonenal.

  18. SNPs not associted with schizophrenia in Japanese individuals

  19. The polymorphism of GCLM -588C/T and -23G/T sites were associated with susceptibility to COPD.

  20. To further investigate the role of the GCLM locus in schizophrenia susceptibility, we studied three SNPs and haplotypes in the region of GCLM using Han Chinese case-control samples.

Mouse (Murine) Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) interaction partners

  1. glutathione levels in the skin of Gclm knock-out mice are reduced by 70 %, which is sufficient for wound repair in young mice, but become rate-limiting upon aging

  2. Gclm(-/-) mice have accelerated ovarian aging due to ovarian oxidative stress.

  3. Upregulation of both GCLC and GLCM mRNA levels in response to cysteine deprivation was dependent on new protein synthesis.

  4. In Gclm KO mice, early-life insults inducing oxidative stress are detrimental to immature parvalbumin interneurons and have long-term consequences.

  5. Wild type female mice deficient in GSH due to deletion of Gclm were resistant to increase visceral adiposity and hepatic steatosis caused by exposure to the PAH BaP.

  6. The glutathione synthesis gene Gclm modulates amphiphilic polymer-coated CdSe/ZnS quantum dot-induced lung inflammation in mice.

  7. Anxiety and drug-stimulated locomotor control brain regions are vulnerable to gclm/glutathione deficiencies.

  8. Clinically relevant levels of TGF-beta1 suppresses GCLC and GCLM expression in mouse lung.

  9. Genetic disruption of Gclm results in reduced aortic glutathione content and impaired acetylcholine mediated aortic ring relaxation.

  10. Data suggest that Gclm is not involved in spermatogenesis but is involved in shielding testis/epididymis from oxidative stress; Gclm knockout mice are fertile but more sensitive to transplacental testicular/epididymal toxicity of benzo[a]pyrene.

  11. gclm KO mice showed a potentiated hyperlocomotor response to amphetamine, impaired sensorimotor gating, and altered social behavior compared to WT. These anomalies resemble important aspects of both SZ and the manic component of BP.

  12. This finding indicates that GSH synthesis can mediate DEP-induced lung inflammation and suggests that polymorphisms in Gclm may be an important factor in determining adverse health outcomes in humans following inhalation of particulate matter.

  13. Gclm is involved in the regulation of basal metabolic rate and metabolism of dietary lipid

  14. investigation of roles for maternal Gclm in preimplantation development: Gclm -/- mice (compared to Gclm +/+ littermates) exhibit lower litter size, fewer uterine implantation sites, delayed blastocyst development after in vitro fertilization

  15. GCLM(-/-) mice were susceptible to myocardial I/R injury partly through an increased vulnerability of mitochondria to oxidative damage owing to mitochondrial GSH reduction

  16. primary fibroblasts from knock-out mice disaplay premature senescence

  17. induction in RAW264.7 cells by Nepsilon-(Carboxymethyl)lysine

  18. Glutamate-cysteine ligase catalytic subunit knockout mice are described.

  19. the oxPAPC-inducible expression of heme oxygenase-1, GCLM, and NQO1 is lower in Nrf2-null than wild-type mouse carotid arteries in vivo.

GCLM Antigen Profile

Protein Summary

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Gene names and symbols associated with GCLM

  • glutamate-cysteine ligase regulatory subunit (PTRG_09814) antibody
  • glutamate cysteine ligase, modifier subunit (Gclm) antibody
  • glutamate-cysteine ligase modifier subunit (GCLM) antibody
  • glutamate-cysteine ligase, modifier subunit (gclm) antibody
  • glutamate-cysteine ligase, modifier subunit L homeolog (gclm.L) antibody
  • glutamate-cysteine ligase, modifier subunit (Gclm) antibody
  • glutamate-cysteine ligase modifier subunit (Gclm) antibody
  • AI649393 antibody
  • Gcmc antibody
  • GLCLR antibody
  • id:ibd3182 antibody
  • wu:fi24c07 antibody
  • zgc:55903 antibody

Protein level used designations for GCLM

glutamate-cysteine ligase regulatory subunit , GCS light chain , gamma-ECS regulatory subunit , gamma-glutamylcysteine synthetase regulatory subunit , glutamate cysteine ligase (gamma-glutamylcysteine synthetase), regulatory , glutamate--cysteine ligase regulatory subunit , glutamate-cysteine ligase modifier subunit , GSC light chain , glutamate--cysteine ligase modifier subunit , glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD) , glutamate-cysteine ligase modifier subunit delta2 alternative splicing , glutamate-cysteine ligase regulatory protein

GENE ID SPECIES
6348110 Pyrenophora tritici-repentis Pt-1C-BFP
29739 Rattus norvegicus
100352174 Oryctolagus cuniculus
333974 Danio rerio
380105 Xenopus laevis
424492 Gallus gallus
2730 Homo sapiens
525659 Bos taurus
14630 Mus musculus
100725164 Cavia porcellus
101117899 Ovis aries
100153977 Sus scrofa
Selected quality suppliers for anti-GCLM (GCLM) Antibodies
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