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Extreme divergence in CENH3 protein sequence does not alter centromere location in Arabidopsis.
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Arabidopsis KNL2 colocalizes with cenH3 and is associated with centromeres during all stages of the mitotic cell cycle, except from metaphase to mid-anaphase.
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Reduced endogenous CENH3 and lack of meiotic incorporation of EYFP-CENH3(C) lead to reduced fertility caused by insufficient CENH3 loading to the centromeres of meiotic chromosomes, subsequent lagging of chromosomes and formation of micronuclei.
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Silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment.
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CENH3 is expressed in dividing tissues and is CENH3 is transcriptionally regulated by E2F/DP transcription factors.
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Deposition of Arabidopsis CENH3 takes place at centromeres during G2.
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Meiosis-specific loading of the centromere-specific histone CENH3 in Arabidopsis thaliana
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study concludes that CENH3 function in A. thaliana, an organism with large tandem repeat centromeres, has stringent requirements for functional complementation in mitosis
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Alignment of CENH3 sequences revealed that the tested species share only three amino acids at loop1 region: threonine2, arginine12 and alanine15
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cenh3 null mutants expressing altered CENH3 proteins are crossed to wild type, chromosomes from the mutant are eliminated, producing haploid progeny