anti-Keratin 12 (KRT12) Antibodies

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Additionally we are shipping Keratin 12 Proteins (8) and Keratin 12 Kits (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
KRT12 3859 Q99456
KRT12 268482  
KRT12 360625 Q6IFW5
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Top anti-Keratin 12 Antibodies at antibodies-online.com

Showing 10 out of 65 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated FACS, IHC (p), WB KRT12 Antibdy (C-term) (ABIN656663) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. Overlay histogram showing K562 cells stained with ABIN2845904 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (ABIN2845904, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Dilution: 1:25 400 μL Log in to see 10 to 11 Days
$385.00
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB KRT12 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT12 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. KRT12 Antibody (C-term) western blot analysis in K562 cell line lysates (35µg/lane).This demonstrates the KRT12 antibody detected the KRT12 protein (arrow). 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Rat Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Human WERI-Rb-1 cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Rat KRT12 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Figure. Western Blot; Sample: Recombinant protein. 100 μg Log in to see 15 to 18 Days
$342.00
Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of KRT12 using Jurkat whole cell lysates Western blot analysis of extracts from 293, using KRT12 Antibody. 100 μL Log in to see 11 to 12 Days
$390.77
Details
Human Rabbit Un-conjugated FACS, IHC (p), WB Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane. 400 μL Log in to see 2 to 3 Days
$515.63
Details
Human Rabbit Un-conjugated IF (p), IHC (p) Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti CK12/Cytokeratin 12 Polyclonal Antibody, Unconjugated (ABIN872955) at 1:200 in 4 °C. Followed by conjugation to secondary antibody at 1:3000 90min in 37 °C. Predicted band 52kD. Observed band size: 52kD. Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-CK12 Polyclonal Antibody, Unconjugated (ABIN872955) at 1:200 followed by conjugation to the secondary antibody and DAB staining 100 μL Log in to see 3 to 7 Days
$317.90
Details
Rat Rabbit Un-conjugated IF/ICC, IHC, IP, WB Western blot analysis of Human WERI-Rb-1 cell lysate, using Rat KRT12 Antibody (1 µg/ml) and HRP-conjugated Goat Anti-Rabbit antibody ( Western blot analysis of the recombinant protein. 100 μg Log in to see 11 to 18 Days
$440.62
Details
Human Guinea Pig Un-conjugated IHC (fro), IF, IHC (p), WB   0.1 mL Log in to see 6 to 8 Days
$489.50
Details
Human Rabbit Un-conjugated IHC (p) Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody - Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in endothelial cells. Immunohistochemistry-Paraffin: Cytokeratin 12 Antibody - Staining of human eye shows strong cytoplasmic positivity in the corneal epithelial cells. 100 μL Log in to see 10 to 13 Days
$494.38
Details
Mouse Rabbit Un-conjugated WB Western blot analysis of extracts of various cell lines, using KRT12 antibody. 200 μL Log in to see 12 to 14 Days
$438.90
Details

Top referenced anti-Keratin 12 Antibodies

  1. Human Polyclonal KRT12 Primary Antibody for FACS, IHC (p) - ABIN656663 : Yu, Bojic, Figueiredo, Rooney, de Havilland, Dickinson, Figueiredo, Lako: An important role for adenine, cholera toxin, hydrocortisone and triiodothyronine in the proliferation, self-renewal and differentiation of limbal stem cells in vitro. in Experimental eye research 2016 (PubMed)
    Show all 2 Pubmed References

  2. Human Polyclonal KRT12 Primary Antibody for IHC (fro), IF - ABIN549050 : Nishida, Adachi, Shimizu-Matsumoto, Kinoshita, Mizuno, Matsubara, Okubo: A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA. in Investigative ophthalmology & visual science 1996 (PubMed)
    Show all 4 Pubmed References

More Antibodies against Keratin 12 Interaction Partners

Human Keratin 12 (KRT12) interaction partners

  1. RT-PCR showed that K3 and K12 transcripts were absent from patient cells, but present in healthy control preparations.

  2. KC can co-exist with GCD. The missense mutation (c.370G > A) in the TGFBI gene and insert mutation (c.1456-1457ins GAT) in the KRT12 gene were identified in a 23-year-old male patient with concurrent KC and GCD.

  3. we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression

  4. Combined with an effective delivery vehicle this siRNA approach represents a viable treatment option for prevention of the MECD pathology observed in K12-Leu132Pro heterozygous individuals.

  5. We identified a novel missense mutation of the KRT12 gene in Meesmann corneal dystrophy. The in vivo confocal microscopy examinations revealed previously unreported depth-dependent ultrastructural changes in the living cornea.

  6. Exon sequencing of KRT3 and KRT12 in six affected and eight unaffected individuals did not detect any mutations or nucleotide sequence variants in Meesmann epithelial corneal dystrophy.

  7. The Leu132Pro missense mutation is within the helix-initiation motif of the keratin and is predicted to result in a significant structural change of the K12 protein.

  8. The lead siRNA, with an IC(50) of thirty picomolar, showed no keratin off-target effects or activation of TLR3 in the concentration ranges tested.

  9. Novel missense mutation within the highly conserved helix-initiation motif of KRT12 causing Meesmann's corneal dystrophy in a German family.

  10. Heterozygous Ala137Pro mutation in keratin 12 gene found in Japanese with Meesmann's corneal dystrophy.

  11. A novel missense mutation (Y429C) in KRT12 lead to MCD in 2 unrelated Taiwanese families.

  12. Mutation in the KRT12 gene is associated with Meesmann corneal dystrophy

  13. Mutation associated with symptomatic phenotype of Meesmann's corneal dystrophy. Results in substitution of proline for arginine in helix termination motif that may disrupt normal helix, leading to dramatic structural change of keratin 12 protein.

  14. This is the second family recently diagnosed with Meesmann dystrophy in Denmark. The family represents its own distinct genotype, independent of previously reported ones. All patients with microcysts were asymptomatic.

  15. The novel L433R mutation of the KRT12 gene found in two members of this Japanese family caused Meesmann corneal dystrophy (MECD).

Mouse (Murine) Keratin 12 (KRT12) interaction partners

  1. data revealed that the early corneal epithelial progenitor cells expressing keratin-12 originated from limbus, and gave rise to the transit amplifying cells that migrated centripetally to differentiate into corneal epithelial cells

  2. clonal limbal stem cells randomly activate Krt12 alleles in the process of terminal differentiation in cornea

Keratin 12 (KRT12) Antigen Profile

Protein Summary

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

Gene names and symbols associated with anti-Keratin 12 (KRT12) Antibodies

  • keratin 91 (krt91) antibody
  • keratin 12 (KRT12) antibody
  • keratin 12 (krt12) antibody
  • keratin 12 (Krt12) antibody
  • keratin 12 S homeolog (krt12.S) antibody
  • AI835216 antibody
  • fb05e09 antibody
  • K12 antibody
  • Ka12 antibody
  • Krt-12 antibody
  • Krt1-12 antibody
  • wu:fa91b04 antibody
  • wu:fa97a07 antibody
  • wu:fb05e09 antibody
  • xk81b2 antibody

Protein level used designations for anti-Keratin 12 (KRT12) Antibodies

keratin 12 , fa97a07 , krtt1c19a , cytokeratin , type I keratin , keratin 12 (Meesmann corneal dystrophy) , CK-12 , cytokeratin-12 , keratin, type I cytoskeletal 12 , keratin-12 , Keratin, type I cytoskeletal 12 (Cytokeratin 12) , keratin complex 1, acidic, gene 12 , K12 , keratin K12 , type I keratin KA12 , keratin, type I cytoskeletal 47 kDa , B2 keratin

GENE ID SPECIES
445051 Danio rerio
100034663 Canis lupus familiaris
100144436 Xenopus (Silurana) tropicalis
468241 Pan troglodytes
3859 Homo sapiens
268482 Mus musculus
360625 Rattus norvegicus
398464 Xenopus laevis
100009289 Oryctolagus cuniculus
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