N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 Proteins (ASAH2)

Human N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) interaction partners

1. The structural basis for ceramide recognition and hydrolysis by human neutral ceramidase has been uncovered.

2. These results demonstrate for the first time that adiponectin inhibits TNFalpha-induced inflammatory response via Cav1-mediated ceramidase recruitment and activation in an AdipoR1-dependent fashion.

3. cloned a 3000 bp region upstream of the translational start site of the nCDase gene

4. c-Jun/AP-1 signaling may, in part, regulate serum-induced nCDase gene transcription

5. Both acidic ceramidase (aCDase) and neutral ceramidase (nCDase) activities declined after low- and high-UVB, but returned to normal only in low-UVB cells

6. Blocking acid ceramidase but not sphingosine kinase activity in alveolar macrophages led to decreased ERK and Akt activity and induction of cell death.

7. Neutral ceramidase is localized mainly in plasma membranes.

8. the present study identified a novel amidase sequence containing a critical serine residue that may function as a nucleophile in the hydrolytic attack on the amide bond present in ceramide

9. Important for the generation of sphgosine 1 phosphqte (S1P) and S1P-mediated cell proliferation and survival.

10. the purification and characterization of neutral ceramidase from human ileostomy content, using octanoyl-[(14)C]sphingosine as substrate

11. serine palmitoyltransferase and ceramidase are two major ceramide metabolizing enzymes that may have roles in psoriatic epidermis

Mouse (Murine) N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) interaction partners

1. These results suggest that neutral ceramidase is a mediator of necroptosis and might be a novel therapeutic target for protection from ischaemic injury.

2. Knocking down NCDase reduced sphingosine accumulation in mitochondria and preserved COX activity after the brain injury.

3. Neutral ceramidase deficiency increases inflammation in a mouse model of inflammatory bowel disease.

4. Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.

5. the predicted HNF-4alpha binding site identified in the Asah2 promoter is functional

6. molecular cloning of the mouse neutral ceramidase gene and its promoter analysis

7. Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1.

8. the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract

9. This enzyme is decreased in the small intestine in CFTR transgenic mice with a deletion in CFTR>