ASAH2 Protein (His tag)
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- Target See all ASAH2 Proteins
- ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ASAH2 protein is labelled with His tag.
- Purpose
- Recombinant Mouse ASAH2 Protein (His Tag)(Active)
- Sequence
- Thr 34-Thr 756
- Characteristics
- A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal polyhistidine tag.
- Purity
- > 97 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.The specific activity is > 3,000 pmoles/min/μg.
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N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 1-756) protein (Strep Tag)
Crystallography grade ASAH2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 1-780) protein (Strep Tag)Crystallography grade ASAH2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 34-780) protein (His tag)Crystallography grade ASAH2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 33-756) protein (His tag)Crystallography grade ASAH2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 99-780) protein (rho-1D4 tag)Crystallography grade ASAH2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 75-756) protein (rho-1D4 tag)Crystallography grade ASAH2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- ASAH2 (N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2))
- Alternative Name
- ASAH2 (ASAH2 Products)
- Synonyms
- BCDase Protein, HNAC1 Protein, LCDase Protein, N-CDase Protein, NCDase Protein, AI585898 Protein, N-acylsphingosine amidohydrolase 2 Protein, ASAH2 Protein, Asah2 Protein
- Background
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Background: ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.
Synonym: AI585898
- Molecular Weight
- 82 kDa
- NCBI Accession
- NP_061300
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