anti-N-Ethylmaleimide-Sensitive Factor (NSF) Antibodies

Required for vesicle-mediated transport. Additionally we are shipping NSF Kits (12) and NSF Proteins (9) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
NSF 4905 P46459
NSF 18195 P46460
NSF 60355 Q9QUL6
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Top anti-NSF Antibodies at antibodies-online.com

Showing 10 out of 116 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-NSF Antibody Titration: 0.2-1 ug/ml Positive Control: Human brain <b>Sample Type</b>: 1. NT-2 cells<br>2. mouse brain extracts<br><b>Primary Antibody Dilution:</b> 2ug/ml<br><b>Secondary Antibody Dilution:</b> 1: 20,000<br><b>Secondary Antibody</b>: IRDye 800CW goat anti-rabbit from Li-COR Bioscience<br><b>Lane 1:</b> 60ug/lane<br><b>Lane 2:</b> 80ug/lane<b><br></b><b>Image Submitted by</b>: Yuzhi Chen<br>University of Arkansas for Medical Science 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated WB Western blot analysis of NSF expression in MCF7 (A), Raw264.7 (B), H9C2 (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Mouse Rabbit Un-conjugated IP, IHC, WB   100 μg Log in to see 2 to 3 Days
$245.00
Details
Human Rabbit Un-conjugated ICC, IHC, WB Figure. Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Mouse Brain Tissue; Lane3: Mouse Testis Tissue. Figure. Western Blot; Sample: Recombinant NSF, Human. 100 μg Log in to see 13 to 16 Days
$350.00
Details
Human Rabbit Un-conjugated WB 0.1 mL Log in to see 6 to 8 Days
$412.50
Details
Mouse Rabbit Un-conjugated IHC, IHC (p), IP, WB Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 7 to 9 Days
$484.00
Details
Human Rabbit Un-conjugated IHC, WB IHC on rat cerebellum (cryo sections) using Rabbit antibody to n-terminal of NSF (Vesicle fusing ATPase): whole serum (ABIN350527) at 1 : 2000 dilution. IHC on rat cerebellum (cryo sections) using Rabbit antibody to n-terminal of NSF (Vesicle fusing ATPase): whole serum (ABIN350527) at 1:2000 dilution. 100 μL Log in to see 7 to 8 Days
$454.67
Details
Rat Mouse Un-conjugated ICC, IP, IHC, ELISA, WB dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2) Indirect immunostaining of hippocampus neurons (dilution 1 : 500) 100 μg Log in to see 5 to 6 Days
$487.03
Details
Cow Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
Details
Mouse Rabbit Un-conjugated WB NSF Antibody (C-term) (ABIN1881589) western blot analysis in mouse cerebellum tissue lysates (35 µg/lane). This demonstrates the NSF antibody detected the NSF protein (arrow). All lanes : Anti-NSF Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Dilution: 1:1000 400 μL Log in to see 10 to 11 Days
$335.50
Details

NSF Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-NSF Antibodies

  1. Human Polyclonal NSF Primary Antibody for ICC, IP - ABIN1742270 : Michaut, Tomes, De Blas, Yunes, Mayorga: Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor. in Proceedings of the National Academy of Sciences of the United States of America 2000 (PubMed)
    Show all 5 Pubmed References

  2. Mouse (Murine) Polyclonal NSF Primary Antibody for WB - ABIN1881589 : Hamza, Zabetian, Tenesa, Laederach, Montimurro, Yearout, Kay, Doheny, Paschall, Pugh, Kusel, Collura, Roberts, Griffith, Samii, Scott, Nutt, Factor, Payami: Common genetic variation in the HLA region is associated with late-onset sporadic Parkinson's disease. in Nature genetics 2010 (PubMed)
    Show all 4 Pubmed References

  3. Rat (Rattus) Monoclonal NSF Primary Antibody for ICC, IP - ABIN1742269 : Ohno, Ishihara, Terada, Kikuta, Sofue, Kawai, Serikawa, Sasa: Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling. in Biochemical and biophysical research communications 2009 (PubMed)
    Show all 2 Pubmed References

More Antibodies against NSF Interaction Partners

Human N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. A large copy number variant (CNV) that encompasses part of the NSF gene contributes to cocaine dependence.

  2. NSF deficiency in HeLa cells barely affected cell viability, anterograde trafficking of vesicular stomatitis virus glycoprotein G and transferrin (show Tf Antibodies) endocytosis.

  3. a processive helicase-like mechanism for NSF in which approximately 1 residue is unwound for every hydrolyzed ATP molecule.

  4. Single and multiple-marker analyses revealed a strong association between cocaine dependence and the NSF gene.

  5. Knockdown of TRX1 (show MLL Antibodies) increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis, and suppresses leukocyte

  6. NSF independent fusion of Salmonella-containing late phagosomes with early endosomes.

  7. analysis of human NSF possible binding mode to GABARAP (show GABARAP Antibodies) and GATE-16 (show GABARAPL2 Antibodies)

  8. binding of NSF to GluR2 (show GRIA2 Antibodies)-containing AMPARs in stabilizing these receptors in the synaptic membrane and impeding their regulated endocytosis.

  9. role of interaction with GluR2 (show GRIA2 Antibodies) in regulation of AMPA (show GRIA3 Antibodies) receptors by brain-derived neurotrophic factor (show BDNF Antibodies)

  10. Nitric oxide regulates exocytosis by S-nitrosylation of NSF.

Mouse (Murine) N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. NSF may have a role in vascular instability in gram-positive lung infections

  2. that mutant LRRK2 may impair synaptic vesicle dynamics via aberrant phosphorylation of N-ethylmaleimide sensitive fusion

  3. identify conserved sequences in the 3'UTR (show UTS2R Antibodies) of NSF as miR (show MLXIP Antibodies)-33 responsive elements and show that Nsf is specifically recruited to the RNA-induced silencing complex following induction of miR (show MLXIP Antibodies)-33

  4. Arr1 (show SAG Antibodies) and NSF interaction are necessary for both maintenance and modulation of normal photoreceptor synaptic regulation and NSF-RP2 interaction may play an important role in membrane protein trafficking in the photoreceptor.

  5. These findings identify new roles for N-ethylmaleimide-sensitive factor and protein kinase C epsilon (show PRKCE Antibodies) in regulating synaptic inhibition through downregulation of GABA(A) receptors

  6. interaction of AMPA (show GRIA3 Antibodies) receptors with N-ethylmaleimide-sensitive factor plays a critical role in incorporation of AMPA (show GRIA3 Antibodies) receptors into synapses and for their subsequent removal during cerebellar long term depression

  7. PICK1 (show PICK1 Antibodies) and NSF dynamically regulate the synaptic delivery of GluR2 (show GRIA2 Antibodies)-containing receptors during CARP (show ANKRD1 Antibodies) and thus regulate the calcium permeability of AMPA (show GRIA3 Antibodies) receptors at excitatory synapses.

  8. H(2)O(2) regulates exocytosis by inhibiting N-ethylmaleimide sensitive factor (NSF), a protein that regulates membrane fusion events necessary for exocytosis.

  9. important role for NSF activity in regulating the cell surface stability of GABA(A) receptors.

  10. dual regulation on hbeta2AR trafficking and signaling by NSF through direct binding to cargo receptor and its ATPase (show DNAH8 Antibodies) activity.

Cow (Bovine) N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. Report depletion of the ATPase (show DNAH8 Antibodies) NSF from Golgi membranes with hypo-S-nitrosylation of vasorelevant proteins in endothelial cells exposed to monocrotaline pyrrole.

Xenopus laevis N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. NSF- and SNARE (show NAPA Antibodies)-mediated membrane fusion events are essential steps during Nuclear envelope formation downstream of Nup107 (show NUP107 Antibodies) recruitment, and upstream of membrane flattening and completion of nuclear pore complex assembly.

Zebrafish N-Ethylmaleimide-Sensitive Factor (NSF) interaction partners

  1. Molecular analysis of ndrg4 (show NDRG4 Antibodies) mutants shows that expression of snap25 (show SNAP25 Antibodies) and nsf are sharply decreased, revealing a role of ndrg4 (show NDRG4 Antibodies) in controlling vesicle exocytosis. This uncovers a previously unknown function of ndrg4 (show NDRG4 Antibodies) in regulating vesicle docking and nodes of Ranvier organization, at least through its ability to finely tune the expression of the t-SNARE (show NAPA Antibodies)/NSF machinery.

  2. results establish a role for Nsf in the maintenance of synaptic contacts between hair cells and afferent neurons, mediated in part via the secretion of trophic signaling factors

  3. Nsf is essential in the formation of the nodes of Ranvier in the vertebrate nervous system.

  4. Activation of PKCgamma is necessary for the activity-dependent trafficking of AMPARs in embryonic zebrafish and is NMDA and SNARE-dependent and requires AMPARs to associate with both NSF and PICK1.

  5. This study analyses of nsf mutant zebrafish reveal an unexpected role for NSF in hypothalamic development, with mutant 5 days post-fertilization larvae exhibiting a stage-dependent loss of neuroendocrine transcripts.

NSF Antigen Profile

Protein Summary

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Gene names and symbols associated with NSF

  • N-ethylmaleimide sensitive factor, vesicle fusing ATPase (NSF) antibody
  • N-ethylmaleimide sensitive fusion protein (Nsf) antibody
  • N-ethylmaleimide sensitive factor, vesicle fusing ATPase (Nsf) antibody
  • N-ethylmaleimide-sensitive factor (NSF) antibody
  • N-ethylmaleimide sensitive factor (nsf) antibody
  • N-ethylmaleimide-sensitive factor (BBOV_II007200) antibody
  • N-ethylmaleimide-sensitive fusion protein, putative (TGME49_318510) antibody
  • N-ethylmaleimide sensitive factor L homeolog (nsf.L) antibody
  • vesicle-fusing ATPase (LOC100180685) antibody
  • vesicle-fusing ATPase (LOC9310886) antibody
  • N-ethylmaleimide-sensitive factor a (nsfa) antibody
  • AAA-type ATPase family protein (NSF) antibody
  • 18.m06598 antibody
  • AI316878 antibody
  • AU020090 antibody
  • AU067812 antibody
  • N-ethylmaleimide sensitive factor antibody
  • NEM-sensitive fusion protein antibody
  • nsf antibody
  • si:bz18k17.1 antibody
  • SKD2 antibody
  • T1J1.4 antibody
  • T1J1_4 antibody
  • wu:fj33g11 antibody

Protein level used designations for NSF

N-ethylmaleimide-sensitive factor-like protein , N-ethylmaleimide-sensitive fusion protein , NEM-sensitive fusion protein , vesicle-fusing ATPase , vesicular-fusion protein NSF , N-ethylmaleimide sensitive factor , suppressor of K(+) transport growth defect 2 , N-ethylmaleimide sensitive fusion protein , N-ethylmaleimide-sensitive factor , vesicle-fusing ATPase-like , Vesicle-fusing ATPase , Vesicular-fusion protein NSF

GENE ID SPECIES
4905 Homo sapiens
18195 Mus musculus
60355 Rattus norvegicus
373397 Strongylocentrotus purpuratus
419972 Gallus gallus
454819 Pan troglodytes
490921 Canis lupus familiaris
504457 Bos taurus
780249 Xenopus (Silurana) tropicalis
5478472 Babesia bovis T2Bo
7901210 Toxoplasma gondii ME49
100024726 Monodelphis domestica
100049722 Xenopus laevis
100063690 Equus caballus
100174078 Pongo abelii
100180685 Ciona intestinalis
100472215 Ailuropoda melanoleuca
779070 Xenopus laevis
9310886 Arabidopsis lyrata subsp. lyrata
368483 Danio rerio
100624659 Sus scrofa
825830 Arabidopsis thaliana
101120344 Ovis aries
100734887 Cavia porcellus
100770898 Cricetulus griseus
715297 Macaca mulatta
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