anti-NHL Repeat Containing 1 (NHLRC1) Antibodies

The protein encoded by NHLRC1 is a single subunit E3 ubiquitin ligase. Additionally we are shipping NHLRC1 Proteins (4) and NHLRC1 Kits (3) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
NHLRC1 378884 Q6VVB1
NHLRC1 105193 Q8BR37
NHLRC1 364682 Q6IMG5
How to order from antibodies-online
  • +1 877 302 8632
  • +1 888 205 9894 (toll-free)
  • Order online
  • orders@antibodies-online.com

Top anti-NHLRC1 Antibodies at antibodies-online.com

Showing 10 out of 68 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Mouse HRP ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$422.40
Details
Human Mouse FITC ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$426.80
Details
Human Mouse Alkaline Phosphatase (AP) ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$429.00
Details
Human Mouse Biotin ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$431.20
Details
Human Mouse PE ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$432.30
Details
Human Mouse Streptavidin ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$433.40
Details
Human Mouse PerCP ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$434.50
Details
Human Mouse APC ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$434.50
Details
Human Mouse Atto 488 ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$435.60
Details
Human Mouse Atto 565 ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Malin Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of ~42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with flag-tagged Malin. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Malin Monoclonal Antibody  at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42 kDa. 100 μg Log in to see 2 to 3 Days
$435.60
Details

NHLRC1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Human ,
,
,
, , , , , , , , , , , , , , , , , , ,
Mouse (Murine)


Rat (Rattus)


Top referenced anti-NHLRC1 Antibodies

  1. Mammalian Monoclonal NHLRC1 Primary Antibody for ISt, IHC - ABIN1304810 : Sengupta, Badhwar, Upadhyay, Singh, Ganesh: Malin and laforin are essential components of a protein complex that protects cells from thermal stress. in Journal of cell science 2011 (PubMed)

More Antibodies against NHLRC1 Interaction Partners

Human NHL Repeat Containing 1 (NHLRC1) interaction partners

  1. Malin promotes its own degradation via auto-ubiquitination.Malin preferentially degrades the phosphatase-inactive laforin (show EPM2A Antibodies) monomer.

  2. laforin/malin complex is able to interact with and ubiquitinate both PKM1 and PKM2

  3. Lafora disease proteins laforin (show EPM2A Antibodies) and malin negatively regulate the HIPK2 (show HIPK2 Antibodies)-p53 (show TP53 Antibodies) cell death pathway.

  4. This study demonistrated that NHLRC1 mutations were detected in some case of Mild Lafora disease patients.

  5. Without functional laforin (show EPM2A Antibodies)-malin complex assembled on polyglucosan bodies, polyglucosan is not degraded.

  6. Malin regulates the recruitment of mRNA-decapping enzyme 1A (Dcp1a (show DCP1A Antibodies)) to processing bodies.

  7. Malin forms a functional complex with laforin (show EPM2A Antibodies). This complex promotes the ubiquitination of proteins involved in glycogen (show GYS1 Antibodies) metabolism and misregulation of pathways involved in this process results in Lafora body formation. (Review)

  8. This study identified that NHLRC1 gene mutations leading to Lafora disease in six Turkish families.

  9. Our results indicate that malin regulates Wnt (show WNT2 Antibodies) signaling pathway through the degradation of dishevelled2 and suggest possible deregulation of Wnt (show WNT2 Antibodies) signaling in Lafora disease.

  10. Mutations in the NHL repeat containing 1 (NHLRC1) gene are described in association with a more benign clinical course and later age of death in an adolescent patient.

Mouse (Murine) NHL Repeat Containing 1 (NHLRC1) interaction partners

  1. The authors conclude that laforin's principle function is to control glycogen (show GYS1 Antibodies) chain lengths, in a malin-dependent fashion, and that loss of this control underlies Lafora disease.

  2. The present study analyzes possible inflammatory responses in the mouse lines Epm2a (-/-) (laforin (show EPM2A Antibodies) knock-out) and Epm2b (-/-) (malin knock-out) with disease progression.

  3. that laforin (show EPM2A Antibodies) and malin are novel regulators of mitochondrial quality control pathway and that the mitochondrial dysfunction resulting from the increased Drp1 (show CRMP1 Antibodies) levels could underlie neuropathology in Lafora disease

  4. Loss of malin leads to reduced proteasomal activity in the heat-shocked cells.

  5. This study also suggests a malin function independent of laforin (show EPM2A Antibodies), possibly in lysosomal biogenesis and/or lysosomal glycogen (show GYS1 Antibodies) disposal.

  6. Results indicate that malin has no effect on whole-body glucose metabolism and insulin (show INS Antibodies) sensitivity.

  7. Dysfunction of autophagy is a common feature of both laforin (show EPM2A Antibodies)- and malin-deficient mice.

  8. malin functions to regulate laforin (show EPM2A Antibodies) and that malin deficiency at least in part causes LB and LD through increased laforin (show EPM2A Antibodies) binding to glycogen (show GYS1 Antibodies).

  9. Results show that a functional laforin (show EPM2A Antibodies)-malin complex plays a critical role in disrupting Lafora bodies and relieving endoplasmic reticulum stres.

  10. Motor coordination, activity impairment, and memory deficits progressively increase with age in Epm2b deficient mice.

NHLRC1 Antigen Profile

Protein Summary

The protein encoded by this gene is a single subunit E3 ubiquitin ligase. Laforin is polyubiquitinated by the encoded protein. Defects in this intronless gene lead to an accumulation of laforin and onset of Lafora disease, also known as progressive myoclonic epilepsy type 2 (EPM2).

Gene names and symbols associated with NHLRC1

  • NHL repeat containing E3 ubiquitin protein ligase 1 (NHLRC1) antibody
  • NHL repeat containing 1 (Nhlrc1) antibody
  • NHL repeat containing E3 ubiquitin protein ligase 1 (Nhlrc1) antibody
  • AI505271 antibody
  • B230309E09Rik antibody
  • bA204B7.2 antibody
  • EPM2A antibody
  • Epm2b antibody
  • MALIN antibody
  • malin-like antibody

Protein level used designations for NHLRC1

E3 ubiquitin-protein ligase NHLRC1 , NHL repeat-containing protein 1 , malin , NHL repeat containing 1 , NHL repeat-containing protein 1-like

GENE ID SPECIES
378884 Homo sapiens
105193 Mus musculus
364682 Rattus norvegicus
428477 Gallus gallus
474161 Canis lupus familiaris
538814 Bos taurus
704451 Macaca mulatta
747581 Pan troglodytes
100066209 Equus caballus
100341052 Oryctolagus cuniculus
100443623 Pongo abelii
Selected quality suppliers for anti-NHLRC1 (NHLRC1) Antibodies
Did you look for something else?