anti-Nuclear Factor I/B (NFIB) Antibodies

Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. Additionally we are shipping NFIB Kits (8) and NFIB Proteins (7) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
NFIB 4781 O00712
NFIB 18028 P97863
NFIB 29227  
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Top anti-NFIB Antibodies at

Showing 10 out of 53 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-NFIB Antibody Titration:  1.25ug/ml  Positive Control:  Transfected 293T 100 μL 2 to 3 Days
Human Rabbit Un-conjugated IHC (p), WB WB Image Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE antibody diluted at 1:500 IHC-P Image NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] , dilution: 1:500. 100 μL 3 to 4 Days
Human Rabbit Un-conjugated IHC, WB Western blot analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: human Hela whole cell lysates,  Lane 2: rat PC-12 whole cell lysates,  Lane 3: mouse lung tissue lysates, Lane 4: mouse ovary tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIB/NF1B2 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIB/NF1B2 at approximately 50KD. The expected band size for NFIB/NF1B2 is at 47KD. IHC analysis of NFIB/NF1B2 using anti-NFIB/NF1B2 antibody . NFIB/NF1B2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NFIB/NF1B2 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4 to 6 Days
Human Mouse Un-conjugated IF, IHC (p), ELISA, WB Immunoperoxidase of monoclonal antibody to NFIB on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Immunofluorescence of monoclonal antibody to NFIB on HeLa cell. [antibody concentration 10 ug/ml] 100 μg 11 to 12 Days
Bat Rabbit Un-conjugated WB 100 μL 11 to 14 Days
Human Rabbit Un-conjugated ICC, IHC, WB Figure. Western Blot; Sample: Recombinant protein. Figure.DAB staining on IHC-P. Samples: Human Tissue 100 μL 13 to 16 Days
Human Rabbit Un-conjugated WB Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane. 400 μL 2 to 3 Days
Human Mouse Un-conjugated ELISA, IHC, IHC (p), WB 100 μg 11 to 14 Days
Human Rabbit Un-conjugated WB 100 μL 11 to 13 Days
Human Rabbit Un-conjugated WB Human NFIB Western blot (WB) 13099 100 μL 14 to 16 Days

Top referenced anti-NFIB Antibodies

  1. Human Polyclonal NFIB Primary Antibody for ICC, IF - ABIN4339322 : Grabowska, Elliott, DeGraff, Anderson, Anumanthan, Yamashita, Sun, Friedman, Hachey, Yu, Sheehan, Ahn, Raj, Piston, Gronostajski, Matusik: NFI transcription factors interact with FOXA1 to regulate prostate-specific gene expression. in Molecular endocrinology (Baltimore, Md.) 2014 (PubMed)
    Show all 4 Pubmed References

  2. Cow (Bovine) Polyclonal NFIB Primary Antibody for WB - ABIN2777544 : Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France. in Diagnostic microbiology and infectious disease 2006 (PubMed)
    Show all 2 Pubmed References

  3. Human Monoclonal NFIB Primary Antibody for IF, IHC (p) - ABIN518322 : Moon, Hwang, Kim, Kim, Lee, Jung, Ko, Han, Noh: NFIB is a potential target for estrogen receptor-negative breast cancers. in Molecular oncology 2011 (PubMed)

More Antibodies against NFIB Interaction Partners

Human Nuclear Factor I/B (NFIB) interaction partners

  1. NFIB promotes cell survival by directly suppressing p21 transcription in TP53-mutated triple-negative breast cancer cells.

  2. High NFIB expression correlates with lymph node metastasis and poor differentiation in tumors of patients with esophagogastric junction adenocarcinoma (EJA) predicting poor outcome.

  3. Breast adenoid cystic carcinomas probably constitute a convergent phenotype, whereby activation of MYB and MYBL1 and their downstream targets can be driven by the MYB-NFIB fusion gene, MYBL1 rearrangements, MYB amplification, or other yet to be identified mechanisms.

  4. This study confirmed the association of SNP rs1324183 in MPDZ-NF1B with keratoconus and revealed the association of this SNP with keratoconus severity and corneal parameters. It is thus a putative genetic marker for monitoring the progression of keratoconus to a severe form and facilitating early intervention.

  5. Haploinsufficiency of NFIB causes ID with macrocephaly.

  6. Nuclear factor I/B (NFIB) is overexpressed in colorectal cancer (CRC) tissues.

  7. Salivary gland ACC cases expressing the MYB-NFIB chimeric gene showed significantly higher blood vessels density compared to non-expressing cases, and suggested that higher VEGF production capability in the former cases may be the cause. The findings also suggested that MYB-NFIB chimeric gene expression may be related to the onset age of ACC.

  8. High NF1B expression is associated with cell growth, aggressiveness, metastasis in gastric cancer.

  9. Both cases harbored the MYB-NFIB gene fusion as demonstrated by FISH and RNA-sequencing

  10. Studied role of miR-30d in tumor invasiveness and migration in non-small lung cancer (NSCLC). Found miR-30d suppressed cell migration and invasion by directly targeting nuclear factor I B (NFIB) in NSCLC.

  11. NFIB-associated gene rearrangement is a frequent genetic event in vulvar adenoid cystic carcinomas. Chromosome translocations involving NFIB but with an intact MYB indicate the presence of novel oncogenic mechanisms for the development of adenoid cystic carcinomas of the vulva.

  12. Study highlighted the paradoxical involvement of NFIB in both the inhibition and promotion of tumor development as well as progression in different malignancies; especially between different tumor subtypes within a single organ system, such as in lung, brain and skin,which corroborates its diverse and distinct roles in specific tissues and cell types. [review]

  13. Expression of the MYB-NFIB fusion oncogene in mammary tissue resulted in hyperplastic glands that developed into adenocarcinoma.

  14. we report that the transcription factor NFIB is associated with significantly improved survival in glioblastoma

  15. A trend toward superior PFS was noted with the MYB/NFIB rearrangement, although this was not statistically significant. NGS revealed three tumors with 4q12 amplification, producing increased copies of axitinib-targeted genes PDGFR/KDR/KIT.

  16. High NFIB levels are associated with expansive growth of a poorly differentiated and almost exclusively E-cadherin (CDH1)-negative invasive tumor cell population.

  17. Studies indicate the role of nuclear factor one (NFIs) as epigenetic regulators in cancer.

  18. low expression of NFIB was significantly associated with biologically more aggressive subtypes and, ultimately, poorer survival in lung adenocarcinoma patients

  19. Data indicate that NFIB protein increases EZH2 protein expression downstream of BRN2 protein, which further decreases MITF protein levels.

  20. Our study defines new molecular subclasses of ACC characterized by MYBL1 rearrangements and 5'-NFIB gene fusions.

Mouse (Murine) Nuclear Factor I/B (NFIB) interaction partners

  1. Results imply that the long noncoding RNA lnc158 positively modulates the transcription level of nuclear factor-IB mRNA, leading to the enhanced induction of oligodendrocytes.

  2. \Nfib hemizygous mice are relatively resistant to hyperoxia compared to wild-type littermates. Nfib hemizygous lungs had significant downregulation of genes involved in redox signaling and inflammatory pathways.

  3. NFIB overexpression interacts with Rb/p53 deletion to promote small cell lung cancer in a mouse model

  4. High NFIB levels are associated with expansive growth of a poorly differentiated and almost exclusively E-cadherin (CDH1)-negative invasive tumor cell population.

  5. Loss of Nfib resulted in a severe delay in intermediate progenitor cell generation while, conversely, overexpression of NFIX led to precocious intermediate progenitor cell generation.

  6. Knockdown of NFIB in Drosha-deficient hippocampal neural stem cells restores neurogenesis, suggesting that the Drosha/NFIB mechanism robustly prevents oligodendrocyte fate acquisition in vivo.

  7. Nfib is necessary and sufficient to increase chromatin accessibility at a large subset of the intergenic regions. Nfib promotes pro-metastatic neuronal gene expression programs and drives the metastatic ability of small cell lung cancer cells.

  8. Loss of Nfib expression resulted in prostatic hyperplasia. NFIB knockdown studies demonstrated that loss of NFIB drives increased AR expression and superinduction of a subset of AR target genes.

  9. Identify regulatory link between Nkx2-1, miR-200c, and the transcription factors Nfib and Myb, adding new players to the regulatory mechanisms driven by Nkx2-1 in lung epithelial cells that may have implications in lung development and tumorigenesis.

  10. NFIA and NFIB are endogenously expressed in gradients with high caudo-medial to low rostro-lateral expression ... this expression pattern deviates from that of PAX6, suggesting that PAX6 does not drive Nfi expression

  11. NFIB-STAT5 modules, possibly in conjunction with other transcription factors, control cell-specific genetic programs.

  12. Study revealed both novel and broad functions of NFIB in regulating the generation of cortical projection neurons, including radial glial differentiation, neuronal migration and axon projection

  13. downregulation of Ezh2 transcription by NFIB is an important component of the process of neural progenitor cell differentiation during cortical development

  14. transcription factor NFIB is an unanticipated coordinator of stem cell behaviour

  15. NFIA and NFIB are expressed in both the developing and the adult olfactory bulb indicative of a regulatory role for these transcription factors in the development of this facet of the olfactory system.

  16. Functional studies indicate that NFIB regulates cell viability and proliferation during transformation

  17. Mesenchymal Nfib regulates both mesenchymal and epithelial cell proliferation through multiple pathways and that mesenchymal NFI-B-mediated signals are essential for the maturation of distal lung epithelium.

  18. Data show that the -588/-560 region of nuclear factor I is involved in the insulin-mediated repression of thyroid stimulating hormone beta.

  19. NFIB/X and NFY repressors contribute differentially to organ-specific regulation of the VWF promoter in transgenic mice.

  20. data indicate that defects in midline glia and cingulate cortex neurons are associated with the callosal dysgenesis seen in Nfib-deficient mice, and provide insight into how the development of these cellular populations is controlled at a molecular level

NFIB Antigen Profile

Protein Summary

Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Gene names and symbols associated with NFIB

  • nuclear factor I B (NFIB) antibody
  • nuclear factor 1 B-type (LOC100221557) antibody
  • nuclear factor I B L homeolog (nfib.L) antibody
  • nuclear factor I/B (Nfib) antibody
  • 6720429L07Rik antibody
  • CTF antibody
  • E030026I10Rik antibody
  • HMGIC/NFIB antibody
  • NF-I/B antibody
  • NF1-B antibody
  • nf1-b1 antibody
  • NFI-B antibody
  • NFI-RED antibody
  • NFIB antibody
  • NFIB2 antibody
  • NFIB3 antibody

Protein level used designations for NFIB

nuclear factor I/B , nuclear factor 1 B-type-like , nuclear factor I-B1 , CCAAT-box binding transcription factor , CCAAT-box-binding transcription factor , CTF , NF-I/B , NF1-B , NFI-B , TGGCA-binding protein , nuclear factor 1 B-type , nuclear factor 1/B , olfactory epithelium nuclear factor I-B

714098 Macaca mulatta
100221557 Taeniopygia guttata
100340884 Oryctolagus cuniculus
100397451 Callithrix jacchus
734209 Xenopus laevis
396209 Gallus gallus
4781 Homo sapiens
474709 Canis lupus familiaris
538474 Bos taurus
18028 Mus musculus
29227 Rattus norvegicus
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