Nuclear RNA Export Factor 1 Proteins (NXF1)

Human Nuclear RNA Export Factor 1 (NXF1) interaction partners

1. NXF1 is involved in coordinating transcriptional dynamics, 3' end processing, and nuclear export of long 3' UTR transcripts, implicating NXF1 as a nexus of gene regulation.

2. Gain or loss-of-function experiments revealed NXF1 selectively regulates TLR7-driven IRF5 transcriptional activity, suggesting a new role for NXF1 in the IRF5 signaling pathway.

3. repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats

4. small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion.

5. Hepatitis B virus core protein associates with the cellular NXF1-p15 complex via the nuclear export signal motif of hepatitis B virus core protein.Nuclear export of human hepatitis B virus core protein and pregenomic RNA depends on the NXF1-p15 machinery.

6. Complementary structural, biochemical and cellular techniques indicated that the formation of a symmetric RNA binding platform generated by dimerization of NXF1:NXT1 facilitates the recognition of CTE-RNA and promotes its nuclear export

7. Gag polyprotein synthesis was decreased by NXF1 knockdown.

8. a spatial map is produced in living cells of the sites for the interaction of two TREX subunits, Alyref and Chtop, with Nxf1.

9. TREX is the major complex used to recruit Nxf1 to mRNA in human cells.

10. the organization of the NXF1 proline-tyrosine nuclear localization signal reveal unexpected redundancy in the nuclear import pathways used by NXF1.

11. The U2AF65 protein served as an adaptor to link expanded CAG RNA to NXF1 for RNA export.

12. The crystal structure of the RNA recognition and leucine-rich repeat motifs of TAP bound to one symmetrical half of the CTE RNA is solved.

13. these data revealed a redox-regulated chaperone function of PDI in delivering antigenic peptides from TAP to MHC-I.

14. Tpr plays an important role in quality control of mRNA trafficked on the Nxf1 pathway.

15. These results showed that the interaction between herpes simplex virus type 1 ICP27 and human TAP/NXF1 occurred in living cells upon head-to-tail intramolecular association of ICP27.

16. Varicella-zoster virus IE4 protein interacts with SR proteins and exports mRNAs through the TAP/NXF1 pathway

17. Depletion of NXF1 or 5,6-dichloro-1-beta-d-ribofuranosyl-benzimidazole treatment had similar effects, inhibiting the nuclear export of several of the H5N1 influenza virus mRNAs.

18. Results describe the subcellular localization of ICP27 and its colocalization with cellular RNA export factors Aly/REF and TAP/NXF1.

19. The structural and mutational data define a novel RNA-binding site on the Tap protein

20. Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1

Mouse (Murine) Nuclear RNA Export Factor 1 (NXF1) interaction partners

1. Mutation E610G, quantitatively accounts for the Nxf1 modifier gene activity toward IAP insertion.

2. Taken together, the results show for the first time that murine leukemia virus requires the Tap/NXF1-mediated export pathway for the expression of its spliced and unspliced RNAs and for full-legth RNA nuclear export.

3. Disruption of NXF1 expression impaired cytoplasmic accumulations of both spliced and unspliced RNA transcripts of Murine leukemia virus.

4. Perturbation of U2AF65/NXF1-mediated RNA nuclear export enhances RNA toxicity in polyQ diseases.

5. RNA transport element RTE evolved as a high affinity RBM15 ligand to provide a splicing-independent link to NXF1, thereby ensuring efficient nuclear export and expression of retrotransposon transcripts

6. FMRP and Nxf2 associate with and destabilize Nxf1 mRNA in neuronal cells.

7. CAST allele of Nxf1 suppresses IAP-Idelta1 insertions in Ap3d1[mh2J], Atcay[hes], Mgrn1[md] and Usp14[axJ]. Does not suppress the Mitf[mi-bw] LINE nor any of six tested ETn insertion alleles.

8. The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1.

9. Allelic variation in Nxf1 suppresses RNA processing defects in IAP insertion alleles of Pitpn (vibrator) and Eya1 (branchio-oto-renal syndrome).